1pue

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[[Image:1pue.gif|left|200px]]<br /><applet load="1pue" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pue, resolution 2.100&Aring;" />
 
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'''PU.1 ETS DOMAIN-DNA COMPLEX'''<br />
 
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==Overview==
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==PU.1 ETS DOMAIN-DNA COMPLEX==
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The Ets family of transcription factors, of which there are now about 35 members regulate gene expression during growth and development. They share a conserved domain of around 85 amino acids which binds as a monomer to the DNA sequence 5'-C/AGGAA/T-3'. We have determined the crystal structure of an ETS domain complexed with DNA, at 2.3-A resolution. The domain is similar to alpha + beta (winged) 'helix-turn-helix' proteins and interacts with a ten-base-pair region of duplex DNA which takes up a uniform curve of 8 degrees. The domain contacts the DNA by a novel loop-helix-loop architecture. Four of amino acids that directly interact with the DNA are highly conserved: two arginines from the recognition helix lying in the major groove, one lysine from the 'wing' that binds upstream of the core GGAA sequence, and another lysine, from the 'turn' of the 'helix-turn-helix' motif, which binds downstream and on the opposite strand.
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<StructureSection load='1pue' size='340' side='right'caption='[[1pue]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1pue]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PUE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PUE FirstGlance]. <br>
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1PUE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PUE OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pue FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pue OCA], [https://pdbe.org/1pue PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pue RCSB], [https://www.ebi.ac.uk/pdbsum/1pue PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pue ProSAT]</span></td></tr>
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==Reference==
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</table>
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A new pattern for helix-turn-helix recognition revealed by the PU.1 ETS-domain-DNA complex., Kodandapani R, Pio F, Ni CZ, Piccialli G, Klemsz M, McKercher S, Maki RA, Ely KR, Nature. 1996 Apr 4;380(6573):456-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8602247 8602247]
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== Disease ==
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[https://www.uniprot.org/uniprot/SPI1_MOUSE SPI1_MOUSE] Note=Involved in murine acute Friend erythroleukemia. It is a target region for SFFV proviral insertion.
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== Function ==
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[https://www.uniprot.org/uniprot/SPI1_MOUSE SPI1_MOUSE] Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.<ref>PMID:8626664</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pu/1pue_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pue ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Synthetic construct]]
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[[Category: Ely, K. R.]]
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[[Category: Ely KR]]
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[[Category: Klemsz, M.]]
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[[Category: Klemsz M]]
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[[Category: Kodandapani, R.]]
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[[Category: Kodandapani R]]
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[[Category: Maki, R A.]]
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[[Category: Maki RA]]
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[[Category: McKercher, S.]]
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[[Category: McKercher S]]
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[[Category: Ni, C Z.]]
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[[Category: Ni CZ]]
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[[Category: Piccialli, G.]]
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[[Category: Piccialli G]]
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[[Category: Pio, F.]]
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[[Category: Pio F]]
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[[Category: activator]]
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[[Category: complex (transcription regulating/dna)]]
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[[Category: dna- binding]]
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[[Category: nuclear protein]]
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[[Category: oncogene]]
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[[Category: transforming protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:32:38 2008''
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Current revision

PU.1 ETS DOMAIN-DNA COMPLEX

PDB ID 1pue

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