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1pvs

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[[Image:1pvs.gif|left|200px]]<br /><applet load="1pvs" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pvs, resolution 2.40&Aring;" />
 
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'''3-methyladenine Glcosylase II(AlkA) Hypoxanthine complex'''<br />
 
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==Overview==
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==3-methyladenine Glcosylase II(AlkA) Hypoxanthine complex==
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Escherichia coli (E. coli) protein 3-methyladenine-DNA glycosylase II (AlkA) functions primarily by removing alkylation damage from duplex and single stranded DNA. A crystal structure of AlkA was refined to 2.0 A resolution. This structure in turn was used to refine an AlkA-hypoxanthine (substrate) complex structure to 2.4 A resolution. The complex structure shows hypoxanthine located in AlkA's active site stacked between residues W218 and Y239. The structural analysis of the AlkA and AlkA-hypoxanthine structures indicate that free hypoxanthine binding in the active site may inhibit glycosylase activity.
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<StructureSection load='1pvs' size='340' side='right'caption='[[1pvs]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1pvs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PVS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7HP:7-HYDROXY-PYRAZOLO[4,3-D]PYRIMIDINE'>7HP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pvs OCA], [https://pdbe.org/1pvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pvs RCSB], [https://www.ebi.ac.uk/pdbsum/1pvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pvs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/3MG2_ECOLI 3MG2_ECOLI] Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2-methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pv/1pvs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pvs ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1PVS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=7HP:'>7HP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-3-methyladenine_glycosylase_II DNA-3-methyladenine glycosylase II], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.21 3.2.2.21] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PVS OCA].
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*[[DNA glycosylase 3D structures|DNA glycosylase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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3-methyladenine-DNA glycosylase II: the crystal structure of an AlkA-hypoxanthine complex suggests the possibility of product inhibition., Teale M, Symersky J, DeLucas L, Bioconjug Chem. 2002 May-Jun;13(3):403-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12009927 12009927]
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[[Category: DNA-3-methyladenine glycosylase II]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Teale, M.]]
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[[Category: Teale M]]
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[[Category: 7HP]]
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[[Category: alka]]
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[[Category: dna glycosylase]]
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[[Category: dna repair]]
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[[Category: hypoxanthine]]
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[[Category: reaction-product complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:33:07 2008''
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Current revision

3-methyladenine Glcosylase II(AlkA) Hypoxanthine complex

PDB ID 1pvs

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