1e99

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[[Image:1e99.png|left|200px]]
 
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{{STRUCTURE_1e99| PDB=1e99 | SCENE= }}
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==Human thymidylate kinase complexed with AZTMP and ADP==
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<StructureSection load='1e99' size='340' side='right'caption='[[1e99]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1e99]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E99 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ATM:3-AZIDO-3-DEOXYTHYMIDINE-5-MONOPHOSPHATE'>ATM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e99 OCA], [https://pdbe.org/1e99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e99 RCSB], [https://www.ebi.ac.uk/pdbsum/1e99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e99 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KTHY_HUMAN KTHY_HUMAN] Catalyzes the conversion of dTMP to dTDP.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e9/1e99_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e99 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 60-fold reduced phosphorylation rate of azidothymidine (AZT) monophosphate (AZTMP), the partially activated AZT metabolite, by human thymidylate kinase (TMPK) severely limits the efficacy of this anti-HIV prodrug. Crystal structures of different TMPK nucleotide complexes indicate that steric hindrance by the azido group of AZTMP prevents formation of the catalytically active closed conformation of the P-loop of TMPK. The F105Y mutant and a chimeric mutant that contains sequences of the human and Escherichia coli enzyme phosphorylate AZTMP 20-fold faster than the wild-type enzyme. The structural basis of the increased activity is assigned to stabilization of the closed P-loop conformation.
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===HUMAN THYMIDYLATE KINASE COMPLEXED WITH AZTMP AND ADP===
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Potentiating AZT activation: structures of wild-type and mutant human thymidylate kinase suggest reasons for the mutants' improved kinetics with the HIV prodrug metabolite AZTMP.,Ostermann N, Lavie A, Padiyar S, Brundiers R, Veit T, Reinstein J, Goody RS, Konrad M, Schlichting I J Mol Biol. 2000 Nov 17;304(1):43-53. PMID:11071809<ref>PMID:11071809</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1e99" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[1e99]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E99 OCA].
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*[[Thymidylate kinase 3D structures|Thymidylate kinase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:011071809</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: DTMP kinase]]
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[[Category: Large Structures]]
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[[Category: Brundiers, R.]]
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[[Category: Brundiers R]]
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[[Category: Goody, R S.]]
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[[Category: Goody RS]]
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[[Category: Konrad, M.]]
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[[Category: Konrad M]]
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[[Category: Lavie, A.]]
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[[Category: Lavie A]]
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[[Category: Ostermann, N.]]
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[[Category: Ostermann N]]
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[[Category: Padiyar, S.]]
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[[Category: Padiyar S]]
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[[Category: Reinstein, J.]]
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[[Category: Reinstein J]]
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[[Category: Schlichting, I.]]
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[[Category: Schlichting I]]
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[[Category: Veit, T.]]
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[[Category: Veit T]]
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[[Category: P-loop]]
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[[Category: Phosphotransferase]]
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[[Category: Thymidylate kinase]]
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Current revision

Human thymidylate kinase complexed with AZTMP and ADP

PDB ID 1e99

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