1pz4

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[[Image:1pz4.jpg|left|200px]]<br /><applet load="1pz4" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pz4, resolution 1.35&Aring;" />
 
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'''The structural determination of an insect (mosquito) Sterol Carrier Protein-2 with a ligand bound C16 Fatty Acid at 1.35 A resolution'''<br />
 
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==Overview==
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==The structural determination of an insect (mosquito) Sterol Carrier Protein-2 with a ligand bound C16 Fatty Acid at 1.35 A resolution==
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Yellow fever mosquito sterol carrier protein (SCP-2) is known to bind to cholesterol. We report here the three-dimensional structure of the complex of SCP-2 from Aedes aegypti with a C16 fatty acid to 1.35-A resolution. The protein fold is exceedingly similar to the human and rabbit proteins, which consist of a five-stranded beta-sheet that exhibits strand order 3-2-1-4-5 with an accompanying layer of four alpha-helices that cover the beta-sheet. A large cavity exists at the interface of the layer alpha-helices and the beta-sheet, which serves as the fatty acid binding site. The carboxylate moiety of the fatty acid is coordinated by a short loop that connects the first alpha-helix to the first beta-strand, whereas the acyl chain extends deep into the interior of the protein. Interestingly, the orientation of the fatty acid is opposite to the observed orientation for Triton X-100 in the SCP-2-like domain from the peroxisomal multifunctional enzyme (Haapalainen, A. M., van Aalten, D. M., Merilainen, G., Jalonen, J. E., Pirila, P., Wierenga, R. K., Hiltunen, J. K., and Glumoff, T. (2001) J. Mol. Biol. 313, 1127-1138). The present study suggests that the binding pocket in the SCP-2 family of proteins may exhibit conformational flexibility to allow coordination of a variety of lipids.
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<StructureSection load='1pz4' size='340' side='right'caption='[[1pz4]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1pz4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aedes_aegypti Aedes aegypti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PZ4 FirstGlance]. <br>
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1PZ4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aedes_aegypti Aedes aegypti] with <scene name='pdbligand=PLM:'>PLM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PZ4 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pz4 OCA], [https://pdbe.org/1pz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pz4 RCSB], [https://www.ebi.ac.uk/pdbsum/1pz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pz4 ProSAT]</span></td></tr>
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The structural determination of an insect sterol carrier protein-2 with a ligand-bound C16 fatty acid at 1.35-A resolution., Dyer DH, Lovell S, Thoden JB, Holden HM, Rayment I, Lan Q, J Biol Chem. 2003 Oct 3;278(40):39085-91. Epub 2003 Jul 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12855689 12855689]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q86PR3_AEDAE Q86PR3_AEDAE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pz/1pz4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pz4 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Aedes aegypti]]
[[Category: Aedes aegypti]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Dyer, D H.]]
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[[Category: Dyer DH]]
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[[Category: Holden, H M.]]
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[[Category: Holden HM]]
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[[Category: Lan, Q.]]
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[[Category: Lan Q]]
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[[Category: Lovell, S.]]
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[[Category: Lovell S]]
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[[Category: Rayment, I.]]
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[[Category: Rayment I]]
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[[Category: Thoden, J B.]]
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[[Category: Thoden JB]]
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[[Category: PLM]]
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[[Category: alpha and beta]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:34:08 2008''
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Current revision

The structural determination of an insect (mosquito) Sterol Carrier Protein-2 with a ligand bound C16 Fatty Acid at 1.35 A resolution

PDB ID 1pz4

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