3m57

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:25, 30 October 2024) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3m57.png|left|200px]]
 
-
{{STRUCTURE_3m57| PDB=3m57 | SCENE= }}
+
==SET7/9 Y245A in complex with TAF10 peptide and AdoHcy==
 +
<StructureSection load='3m57' size='340' side='right'caption='[[3m57]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3m57]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M57 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M57 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m57 OCA], [https://pdbe.org/3m57 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m57 RCSB], [https://www.ebi.ac.uk/pdbsum/3m57 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m57 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/SETD7_HUMAN SETD7_HUMAN] Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.<ref>PMID:12588998</ref> <ref>PMID:15099517</ref> <ref>PMID:16141209</ref> <ref>PMID:17108971</ref> <ref>PMID:12540855</ref> <ref>PMID:15525938</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m5/3m57_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3m57 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
SET domain lysine methyltransferases (KMTs) methylate specific lysine residues in histone and non-histone substrates. These enzymes also display product specificity by catalyzing distinct degrees of methylation of the lysine epsilon-amino group. To elucidate the molecular mechanism underlying this specificity, we have characterized the Y245A and Y305F mutants of the human KMT SET7/9 (also known as KMT7) that alter its product specificity from a monomethyltransferase to a di- and a trimethyltransferase, respectively. Crystal structures of these mutants in complex with peptides bearing unmodified, mono-, di-, and trimethylated lysines illustrate the roles of active site water molecules in aligning the lysine epsilon-amino group for methyl transfer with S-adenosylmethionine. Displacement or dissociation of these solvent molecules enlarges the diameter of the active site, accommodating the increasing size of the methylated epsilon-amino group during successive methyl transfer reactions. Together, these results furnish new insights into the roles of active site water molecules in modulating lysine multiple methylation by SET domain KMTs and provide the first molecular snapshots of the mono-, di-, and trimethyl transfer reactions catalyzed by these enzymes.
-
===SET7/9 Y245A in complex with TAF10 peptide and AdoHcy===
+
SET7/9 catalytic mutants reveal the role of active site water molecules in lysine multiple methylation.,Del Rizzo PA, Couture JF, Dirk LM, Strunk BS, Roiko MS, Brunzelle JS, Houtz RL, Trievel RC J Biol Chem. 2010 Oct 8;285(41):31849-58. Epub 2010 Aug 1. PMID:20675860<ref>PMID:20675860</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 3m57" style="background-color:#fffaf0;"></div>
-
==About this Structure==
+
==See Also==
-
[[3m57]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M57 OCA].
+
*[[Histone methyltransferase 3D structures|Histone methyltransferase 3D structures]]
-
[[Category: Histone-lysine N-methyltransferase]]
+
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Brunzelle, J S.]]
+
[[Category: Large Structures]]
-
[[Category: Couture, J F.]]
+
[[Category: Brunzelle JS]]
-
[[Category: Dirk, L M.]]
+
[[Category: Couture J-F]]
-
[[Category: Houtz, R L.]]
+
[[Category: Del Rizzo PA]]
-
[[Category: Rizzo, P A.Del.]]
+
[[Category: Dirk LM]]
-
[[Category: Roiko, M S.]]
+
[[Category: Houtz RL]]
-
[[Category: Strunk, B S.]]
+
[[Category: Roiko MS]]
-
[[Category: Trievel, R C.]]
+
[[Category: Strunk BS]]
-
[[Category: Chromatin regulator]]
+
[[Category: Trievel RC]]
-
[[Category: Chromosomal protein]]
+
-
[[Category: Methyltransferase]]
+
-
[[Category: Nucleus]]
+
-
[[Category: S-adenosyl-l-homocysteine]]
+
-
[[Category: S-adenosyl-l-methionine]]
+
-
[[Category: Set domain]]
+
-
[[Category: Taf10 peptide]]
+
-
[[Category: Ternary complex]]
+
-
[[Category: Transcription]]
+
-
[[Category: Transcription regulation]]
+
-
[[Category: Transferase]]
+

Current revision

SET7/9 Y245A in complex with TAF10 peptide and AdoHcy

PDB ID 3m57

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3m57"
Personal tools