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4hxf
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4hxf is ON HOLD Authors: Kiss-Szeman, A., Menyhard, D.K., Tichy-Racs, E., Hornung, B., Radi, K., Szeltner, Z., Domokos, K., Szamosi, I., Naray-Szabo...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Acylaminoacyl peptidase in complex with Z-Gly-Gly-Phe-chloromethyl ketone== | |
| + | <StructureSection load='4hxf' size='340' side='right'caption='[[4hxf]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4hxf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HXF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HXF FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.601Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=Y3A:N-[(BENZYLOXY)CARBONYL]GLYCYL-N-[(2S,3R)-4-CHLORO-3-HYDROXY-1-PHENYLBUTAN-2-YL]GLYCINAMIDE'>Y3A</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hxf OCA], [https://pdbe.org/4hxf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hxf RCSB], [https://www.ebi.ac.uk/pdbsum/4hxf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hxf ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/O58323_PYRHO O58323_PYRHO] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Oligopeptidases impose a size limitation on their substrates, the mechanism of which has long been in debate. Here we present the structure of a hexameric serine protease, an oligopeptidase from Pyrococcus horikoshii (PhAAP), revealing a complex, self-compartmentalized inner space, where substrates may access the monomer active sites passing through a double-gated "check-in" system: first passing through a pore on the hexamer surface, then turning to enter through an even smaller opening at the monomers' domain-interface. This substrate screening strategy is unique within the family. We found that among oligopeptidases a member of catalytic apparatus is positioned near an amylogenic beta-edge, which needs to be protected to prevent aggregation and found different strategies applied to such end. We propose that self-assembly within the family results in characteristically different substrate selection mechanisms coupled to different multimerization states. | ||
| - | + | A self-compartmentalizing hexamer serine protease from Pyrococcus horikoshii - substrate selection achieved through multimerization.,Menyhard DK, Kiss-Szeman A, Tichy-Racs E, Hornung B, Radi K, Szeltner Z, Domokos K, Szamosi I, Naray-Szabo G, Polgar L, Harmat V J Biol Chem. 2013 Apr 30. PMID:23632025<ref>PMID:23632025</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4hxf" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Acylaminoacyl peptidase 3D structures|Acylaminoacyl peptidase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pyrococcus horikoshii OT3]] | ||
| + | [[Category: Domokos K]] | ||
| + | [[Category: Harmat V]] | ||
| + | [[Category: Hornung B]] | ||
| + | [[Category: Kiss-Szeman A]] | ||
| + | [[Category: Menyhard DK]] | ||
| + | [[Category: Naray-Szabo G]] | ||
| + | [[Category: Polgar L]] | ||
| + | [[Category: Radi K]] | ||
| + | [[Category: Szamosi I]] | ||
| + | [[Category: Szeltner Z]] | ||
| + | [[Category: Tichy-Racs E]] | ||
Current revision
Acylaminoacyl peptidase in complex with Z-Gly-Gly-Phe-chloromethyl ketone
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