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4hz7
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4hz7 is ON HOLD Authors: Hwang, K.Y., Nam, K.H. Description: Crystal structure of BglB with glucose) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of BglB with glucose== | |
| + | <StructureSection load='4hz7' size='340' side='right'caption='[[4hz7]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4hz7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium Uncultured bacterium]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3fiz 3fiz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HZ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HZ7 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hz7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hz7 OCA], [https://pdbe.org/4hz7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hz7 RCSB], [https://www.ebi.ac.uk/pdbsum/4hz7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hz7 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q0GMU3_9BACT Q0GMU3_9BACT] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Beta-glucosidase enzymes (EC 3.2.1-3.2.3) hydrolyze sugars and are implicated in a wide spectrum of biological processes. Recently, we reported that beta-glucosidase has varied kinetic parameters for the natural and synthetic substrates [K.H Nam, S.J. Kim, M.Y. Kim, J.H. Kim, T.S. Yeo, C.M. Lee, H.K Jun, K.Y. Hwang. Crystal structure of engineered beta-glucosidase from a soil metagenome, Proteins 73 (2008) 788-793]. However, an understanding of the kinetic values of beta-glucosidase has not yet enabled the elucidation of its molecular function. Here, we report the X-ray crystal structure of beta-glucosidase with a glucose and cellobiose fragment from uncultured soil metagenome. From the various crystals, we obtained the pre-reaction (native), intermediate (disaccharide cleavage) and post-reaction (glucose binding) states of the active site pocket. These structures provide snapshots of the catalytic processing of beta-glucosidase. In addition, the intermediate state of the crystal structure provides insight into the substrate specificity of beta-glucosidase. These structural studies will facilitate elucidation of the architectural mechanism responsible for the substrate recognition of beta-glucosidase. | ||
| - | + | Structural insights into the substrate recognition properties of beta-glucosidase.,Nam KH, Sung MW, Hwang KY Biochem Biophys Res Commun. 2010 Jan 1;391(1):1131-5. doi:, 10.1016/j.bbrc.2009.12.038. Epub 2009 Dec 11. PMID:20005197<ref>PMID:20005197</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4hz7" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Uncultured bacterium]] | ||
| + | [[Category: Hwang KY]] | ||
| + | [[Category: Nam KH]] | ||
Current revision
Crystal structure of BglB with glucose
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