1ql2

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[[Image:1ql2.gif|left|200px]]<br /><applet load="1ql2" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ql2, resolution 3.1&Aring;" />
 
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'''INOVIRUS (FILAMENTOUS BACTERIOPHAGE) STRAIN PF1 MAJOR COAT PROTEIN ASSEMBLY'''<br />
 
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==Overview==
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==Inovirus (Filamentous Bacteriophage) Strain PF1 Major Coat Protein Assembly==
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The major coat protein in the capsid of Pf1 filamentous bacteriophage (Inovirus) forms a helical assembly of about 7000 identical protein subunits, each of which contains 46 amino-acid residues and can be closely approximated by a single gently curved alpha-helix. Since the viral DNA occupies the core of the tubular capsid and appears to make no significant specific interactions with the capsid proteins, the capsid is a simple model system for the study of the static and dynamic properties of alpha-helix assembly. The capsid undergoes a reversible temperature-induced structural transition at about 283 K between two slightly different helix forms. The two forms can coexist without an intermediate state, consistent with a first-order structural phase transition. The molecular model of the higher temperature form was refined using improved X-ray fibre diffraction data and new refinement and validation methods. The refinement indicates that the two forms are related by a change in the orientation of the capsid subunits within the virion, without a significant change in local conformation of the subunits. On the higher temperature diffraction pattern there is a region of observed intensity that is not consistent with a simple helix of identical subunits; it is proposed that the structure involves groups of three subunits which each have a slightly different orientation within the group. The grouping of subunits suggests that a change in subunit libration frequency could be the basis of the Pf1 structural transition; calculations from the model are used to explore this idea.
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<StructureSection load='1ql2' size='340' side='right'caption='[[1ql2]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ql2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_Pf1 Pseudomonas virus Pf1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QL2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QL2 FirstGlance]. <br>
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1QL2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_pf1 Pseudomonas phage pf1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QL2 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Fiber diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ql2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ql2 OCA], [https://pdbe.org/1ql2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ql2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ql2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ql2 ProSAT]</span></td></tr>
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==Reference==
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</table>
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The molecular structure and structural transition of the alpha-helical capsid in filamentous bacteriophage Pf1., Welsh LC, Symmons MF, Marvin DA, Acta Crystallogr D Biol Crystallogr. 2000 Feb;56(Pt 2):137-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10666593 10666593]
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== Function ==
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[[Category: Pseudomonas phage pf1]]
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[https://www.uniprot.org/uniprot/CAPSD_BPPF1 CAPSD_BPPF1] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity).
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[[Category: Single protein]]
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__TOC__
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[[Category: Marvin, D A.]]
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</StructureSection>
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[[Category: Symmons, M F.]]
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[[Category: Large Structures]]
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[[Category: Welsh, L C.]]
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[[Category: Pseudomonas virus Pf1]]
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[[Category: helical virus]]
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[[Category: Marvin DA]]
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[[Category: helical virus coat protein]]
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[[Category: Symmons MF]]
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[[Category: inovirus]]
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[[Category: Welsh LC]]
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[[Category: ssdna viruses]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:40:50 2008''
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Current revision

Inovirus (Filamentous Bacteriophage) Strain PF1 Major Coat Protein Assembly

PDB ID 1ql2

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