1fh5

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[[Image:1fh5.png|left|200px]]
 
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{{STRUCTURE_1fh5| PDB=1fh5 | SCENE= }}
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==CRYSTAL STRUCTURE OF THE FAB FRAGMENT OF THE MONOCLONAL ANTIBODY MAK33==
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<StructureSection load='1fh5' size='340' side='right'caption='[[1fh5]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1fh5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FH5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FH5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fh5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fh5 OCA], [https://pdbe.org/1fh5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fh5 RCSB], [https://www.ebi.ac.uk/pdbsum/1fh5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fh5 ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fh/1fh5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fh5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Fab fragment of the murine monoclonal antibody, MAK33, directed against human creatine kinase of the muscle-type, was crystallized and the three-dimensional structure was determined to 2.9 A. The antigen-binding surface of MAK33 shows a convex overall shape typical for immunoglobulins binding large antigens. The structure allows us to analyze the environment of cis-prolyl-peptide bonds whose isomerization is of key importance in the folding process. These residues seem to be involved with not only domain stability but also seem to play a role in the association of heavy and light chains, reinforcing the importance of beta-strand recognition in antibody assembly. The structure also allows the localization of segments of primary sequence postulated to represent binding sites for the ER-specific chaperone BiP within the context of the entire Fab fragment. These sequences are found primarily in beta-strands that are necessary for interactions between the individual domains.
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===CRYSTAL STRUCTURE OF THE FAB FRAGMENT OF THE MONOCLONAL ANTIBODY MAK33===
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The crystal structure of the fab fragment of the monoclonal antibody MAK33. Implications for folding and interaction with the chaperone bip.,Augustine JG, de La Calle A, Knarr G, Buchner J, Frederick CA J Biol Chem. 2001 Feb 2;276(5):3287-94. Epub 2000 Oct 17. PMID:11036070<ref>PMID:11036070</ref>
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{{ABSTRACT_PUBMED_11036070}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1fh5" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[1fh5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FH5 OCA].
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:011036070</ref><references group="xtra"/>
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__TOC__
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[[Category: Homo sapiens]]
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</StructureSection>
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[[Category: Augustine, J G.]]
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[[Category: Large Structures]]
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[[Category: Buchner, J.]]
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[[Category: Mus musculus]]
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[[Category: Calle, A de la.]]
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[[Category: Augustine JG]]
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[[Category: Frederick, C A.]]
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[[Category: Buchner J]]
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[[Category: Knarr, G.]]
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[[Category: Frederick CA]]
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[[Category: Bip]]
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[[Category: Knarr G]]
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[[Category: Fab]]
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[[Category: De la Calle A]]
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[[Category: Immune system]]
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Current revision

CRYSTAL STRUCTURE OF THE FAB FRAGMENT OF THE MONOCLONAL ANTIBODY MAK33

PDB ID 1fh5

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