1hl7

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[[Image:1hl7.png|left|200px]]
 
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{{STRUCTURE_1hl7| PDB=1hl7 | SCENE= }}
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==Gamma lactamase from an Aureobacterium species in complex with 3a,4,7,7a-tetrahydro-benzo [1,3] dioxol-2-one==
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<StructureSection load='1hl7' size='340' side='right'caption='[[1hl7]], [[Resolution|resolution]] 1.73&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1hl7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Microbacterium_sp. Microbacterium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HL7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HL7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.73&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BD1:3A,4,7,7A-TETRAHYDRO-BENZO+[1,3]+DIOXOL-2-ONE'>BD1</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hl7 OCA], [https://pdbe.org/1hl7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hl7 RCSB], [https://www.ebi.ac.uk/pdbsum/1hl7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hl7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8GJP7_9MICO Q8GJP7_9MICO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/1hl7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hl7 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of the recombinant (-) gamma-lactamase from an Aureobacterium species has been solved at 1.73A resolution in the cubic space group F23 with unit cell parameters a=b=c=240.6A. The trimeric enzyme has an alpha/beta hydrolase fold and closely resembles the cofactor free haloperoxidases. The structure has been solved in complex with a covalently bound ligand originating from the host cell and also in the unligated form. The associated density in the former structure has been interpreted as the two-ring ligand (3aR,7aS)-3a,4,7,7a-tetrahydro-benzo [1,3] dioxol-2-one which forms a tetrahedral complex with OG of the catalytic Ser98. Soaks of these crystals with the industrial substrate gamma-lactam or its structural analogue, norcamphor, result in the displacement of the ligand from the enzyme active site, thereby allowing determination of the unligated structure. The presence of the ligand in the active site protects the enzyme from serine hydrolase inhibitors. Cyclic ethylene carbonate, the first ring of the ligand, was shown to be a substrate of the enzyme.
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===GAMMA LACTAMASE FROM AN AUREOBACTERIUM SPECIES IN COMPLEX WITH 3A,4,7,7A-TETRAHYDRO-BENZO [1,3] DIOXOL-2-ONE===
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The crystal structure of a (-) gamma-lactamase from an Aureobacterium species reveals a tetrahedral intermediate in the active site.,Line K, Isupov MN, Littlechild JA J Mol Biol. 2004 Apr 30;338(3):519-32. PMID:15081810<ref>PMID:15081810</ref>
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{{ABSTRACT_PUBMED_15081810}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1hl7" style="background-color:#fffaf0;"></div>
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[[1hl7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Microbacterium_sp. Microbacterium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HL7 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:015081810</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Microbacterium sp.]]
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[[Category: Large Structures]]
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[[Category: Isupov, M N.]]
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[[Category: Microbacterium sp]]
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[[Category: Line, K.]]
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[[Category: Isupov MN]]
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[[Category: Littlechild, J A.]]
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[[Category: Line K]]
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[[Category: Alpha/beta hydrolase]]
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[[Category: Littlechild JA]]
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[[Category: Co-factor free haloperoxidase]]
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[[Category: Hydrolase]]
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[[Category: Tetrahedral intermediate]]
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Current revision

Gamma lactamase from an Aureobacterium species in complex with 3a,4,7,7a-tetrahydro-benzo [1,3] dioxol-2-one

PDB ID 1hl7

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