1qto

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[[Image:1qto.jpg|left|200px]]<br /><applet load="1qto" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qto, resolution 1.5&Aring;" />
 
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'''1.5 A CRYSTAL STRUCTURE OF A BLEOMYCIN RESISTANCE DETERMINANT FROM BLEOMYCIN-PRODUCING STREPTOMYCES VERTICILLUS'''<br />
 
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==Overview==
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==1.5 A CRYSTAL STRUCTURE OF A BLEOMYCIN RESISTANCE DETERMINANT FROM BLEOMYCIN-PRODUCING STREPTOMYCES VERTICILLUS==
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Bleomycin (Bm)-binding protein, designated BLMA, which is a Bm resistance determinant from Bm-producing Streptomyces verticillus, was crystallized in a form suitable for X-ray diffraction analysis. The diffraction intensity data were collected up to a resolution of 1.5 A with a merging R-value of 0.054 at a completeness of 94 %. The BLMA structure, determined by the single isomorphous replacement method including the anomalous scattering effect (SIR-AS) at a resolution of 2.0 A, was refined at 1.5 A resolution. The final R-factor was 19.0 % and R(free) was 22.1 % including 91 water molecules. The crystal packing showed a dimer form, which was generated by arm exchange. The 1.5 A high-resolution experiment allowed an analysis of the side-chain disorder of BLMA. The structural comparison of BLMA with a homologous protein from Streptoalloteichus hindustanus, designated Shble protein, showed that a Ser100-Gly103 loop was farther from the groove, which is a Bm-binding site, in BLMA than in the Shble protein. Furthermore the hydrophobicity of the groove in BLMA is much lower than that in the Shble protein. The structural differences between these proteins may be responsible for the observation that a half-saturating concentration (K(1/2)) of Bm is higher for BLMA than for the Shble protein.
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<StructureSection load='1qto' size='340' side='right'caption='[[1qto]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1qto]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_verticillus Streptomyces verticillus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QTO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QTO FirstGlance]. <br>
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1QTO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_verticillus Streptomyces verticillus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QTO OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qto FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qto OCA], [https://pdbe.org/1qto PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qto RCSB], [https://www.ebi.ac.uk/pdbsum/1qto PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qto ProSAT]</span></td></tr>
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==Reference==
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</table>
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The 1.5 A crystal structure of a bleomycin resistance determinant from bleomycin-producing Streptomyces verticillus., Kawano Y, Kumagai T, Muta K, Matoba Y, Davies J, Sugiyama M, J Mol Biol. 2000 Jan 28;295(4):915-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10656800 10656800]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/Q53793_9ACTN Q53793_9ACTN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qt/1qto_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qto ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces verticillus]]
[[Category: Streptomyces verticillus]]
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[[Category: Davies, J.]]
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[[Category: Davies J]]
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[[Category: Kawano, Y.]]
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[[Category: Kawano Y]]
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[[Category: Kumagai, T.]]
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[[Category: Kumagai T]]
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[[Category: Matoba, Y.]]
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[[Category: Matoba Y]]
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[[Category: Muta, K.]]
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[[Category: Muta K]]
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[[Category: Sugiyama, M.]]
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[[Category: Sugiyama M]]
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[[Category: arm-exchange]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:43:32 2008''
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Current revision

1.5 A CRYSTAL STRUCTURE OF A BLEOMYCIN RESISTANCE DETERMINANT FROM BLEOMYCIN-PRODUCING STREPTOMYCES VERTICILLUS

PDB ID 1qto

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