1qv4

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[[Image:1qv4.gif|left|200px]]<br /><applet load="1qv4" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qv4, resolution 2.5&Aring;" />
 
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'''B-DNA Dodecamer CGTGAATTCACG complexed with minor groove binder methylproamine'''<br />
 
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==Overview==
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==B-DNA Dodecamer CGTGAATTCACG complexed with minor groove binder methylproamine==
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New analogues of the minor groove binding ligand Hoechst 33342 have been investigated in an attempt to improve radioprotective activity. The synthesis, DNA binding, and in vitro radioprotective properties of methylproamine, the most potent derivative, are reported. Experiments with V79 cells have shown that methylproamine is approximately 100-fold more potent than the classical aminothiol radioprotector WR1065. The crystal structures of methylproamine and proamine complexes with the dodecamer d(CGCGAATTCGCG)(2) confirm that the new analogues also are minor groove binders. It is proposed that the DNA-bound methylproamine ligand acts as a reducing agent by an electron transfer mechanism, repairing transient radiation-induced oxidizing species on DNA.
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<StructureSection load='1qv4' size='340' side='right'caption='[[1qv4]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1qv4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QV4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QV4 FirstGlance]. <br>
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1QV4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MBB:'>MBB</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QV4 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MBB:2-(3-METHYL-4-DIMETHYLAMINOPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5-BI-BENZIMIDAZOLE'>MBB</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qv4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qv4 OCA], [https://pdbe.org/1qv4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qv4 RCSB], [https://www.ebi.ac.uk/pdbsum/1qv4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qv4 ProSAT]</span></td></tr>
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In vitro studies with methylproamine: a potent new radioprotector., Martin RF, Broadhurst S, Reum ME, Squire CJ, Clark GR, Lobachevsky PN, White JM, Clark C, Sy D, Spotheim-Maurizot M, Kelly DP, Cancer Res. 2004 Feb 1;64(3):1067-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14871839 14871839]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Clark, G R.]]
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</StructureSection>
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[[Category: Squire, C J.]]
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[[Category: Large Structures]]
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[[Category: MBB]]
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[[Category: Clark GR]]
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[[Category: b-dna]]
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[[Category: Squire CJ]]
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[[Category: minor groove binder]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:44:05 2008''
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Current revision

B-DNA Dodecamer CGTGAATTCACG complexed with minor groove binder methylproamine

PDB ID 1qv4

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