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1qwj

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[[Image:1qwj.gif|left|200px]]<br /><applet load="1qwj" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qwj, resolution 2.8&Aring;" />
 
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'''The Crystal Structure of Murine CMP-5-N-Acetylneuraminic Acid Synthetase'''<br />
 
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==Overview==
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==The Crystal Structure of Murine CMP-5-N-Acetylneuraminic Acid Synthetase==
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Sialic acids are activated by CMP-5-N-acetylneuraminic acid synthetase prior to their transfer onto oligo- or polysaccharides. Here, we present the crystal structure of the N-terminal catalytically active domain of the murine 5-N-acetylneuraminic acid synthetase in complex with the reaction product. In contrast to the previously solved structure of 5-N-acetylneuraminic acid synthetase from Neisseria meningitidis and the related CMP-KDO-synthetase of Escherichia coli, the murine enzyme is a tetramer, which was observed with the active sites closed. In this conformation a loop is shifted by 6A towards the active site and thus an essential arginine residue can participate in catalysis. Furthermore, a network of intermolecular salt-bridges and hydrogen bonds in the dimer as well as hydrophobic interfaces between two dimers indicate a cooperative behaviour of the enzyme. In addition, a complex regulation of the enzyme activity is proposed that includes phosphorylation and dephosphorylation.
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<StructureSection load='1qwj' size='340' side='right'caption='[[1qwj]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1qwj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QWJ FirstGlance]. <br>
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1QWJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NCC:'>NCC</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/N-acylneuraminate_cytidylyltransferase N-acylneuraminate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.43 2.7.7.43] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWJ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCC:CYTIDINE-5-MONOPHOSPHATE-5-N-ACETYLNEURAMINIC+ACID'>NCC</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwj OCA], [https://pdbe.org/1qwj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qwj RCSB], [https://www.ebi.ac.uk/pdbsum/1qwj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qwj ProSAT]</span></td></tr>
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The crystal structure of murine CMP-5-N-acetylneuraminic acid synthetase., Krapp S, Munster-Kuhnel AK, Kaiser JT, Huber R, Tiralongo J, Gerardy-Schahn R, Jacob U, J Mol Biol. 2003 Dec 5;334(4):625-37. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14636592 14636592]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NEUA_MOUSE NEUA_MOUSE] Catalyzes the activation of N-acetylneuraminic acid (NeuNAc) to cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuNAc), a substrate required for the addition of sialic acid. Has some activity toward NeuNAc, N-glycolylneuraminic acid (Neu5Gc) or 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN).<ref>PMID:9689047</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qw/1qwj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qwj ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: N-acylneuraminate cytidylyltransferase]]
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[[Category: Gerardy-Schahn R]]
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[[Category: Single protein]]
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[[Category: Huber R]]
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[[Category: Gerardy-Schahn, R.]]
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[[Category: Jacob U]]
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[[Category: Huber, R.]]
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[[Category: Kaiser JT]]
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[[Category: Jacob, U.]]
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[[Category: Krapp S]]
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[[Category: Kaiser, J T.]]
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[[Category: Muenster-Kuehnel AK]]
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[[Category: Krapp, S.]]
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[[Category: Tiralongo J]]
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[[Category: Muenster-Kuehnel, A K.]]
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[[Category: Tiralongo, J.]]
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[[Category: NCC]]
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[[Category: cmp-5-n-acetylneuraminic acid synthetase]]
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[[Category: cmp-neu5ac]]
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[[Category: glycosylation]]
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[[Category: lipopolysaccharide biosynthesis]]
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[[Category: sialic acid]]
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[[Category: sugar-activating enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:44:27 2008''
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Current revision

The Crystal Structure of Murine CMP-5-N-Acetylneuraminic Acid Synthetase

PDB ID 1qwj

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