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1qzb

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[[Image:1qzb.gif|left|200px]]<br /><applet load="1qzb" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qzb" />
 
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'''Coordinates of the A-site tRNA model fitted into the cryo-EM map of 70S ribosome in the pre-translocational state'''<br />
 
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==Overview==
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==Coordinates of the A-site tRNA model fitted into the cryo-EM map of 70S ribosome in the pre-translocational state==
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Aminoacyl-tRNAs (aa-tRNAs) are delivered to the ribosome as part of the ternary complex of aa-tRNA, elongation factor Tu (EF-Tu) and GTP. Here, we present a cryo-electron microscopy (cryo-EM) study, at a resolution of approximately 9 A, showing that during the incorporation of the aa-tRNA into the 70S ribosome of Escherichia coli, the flexibility of aa-tRNA allows the initial codon recognition and its accommodation into the ribosomal A site. In addition, a conformational change observed in the GTPase-associated center (GAC) of the ribosomal 50S subunit may provide the mechanism by which the ribosome promotes a relative movement of the aa-tRNA with respect to EF-Tu. This relative rearrangement seems to facilitate codon recognition by the incoming aa-tRNA, and to provide the codon-anticodon recognition-dependent signal for the GTPase activity of EF-Tu. From these new findings we propose a mechanism that can explain the sequence of events during the decoding of mRNA on the ribosome.
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<SX load='1qzb' size='340' side='right' viewer='molstar' caption='[[1qzb]], [[Resolution|resolution]] 9.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1qzb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QZB FirstGlance]. <br>
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1QZB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZB OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qzb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzb OCA], [https://pdbe.org/1qzb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qzb RCSB], [https://www.ebi.ac.uk/pdbsum/1qzb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qzb ProSAT]</span></td></tr>
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==Reference==
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</table>
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Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy., Valle M, Zavialov A, Li W, Stagg SM, Sengupta J, Nielsen RC, Nissen P, Harvey SC, Ehrenberg M, Frank J, Nat Struct Biol. 2003 Nov;10(11):899-906. Epub 2003 Oct 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14566331 14566331]
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__TOC__
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[[Category: Protein complex]]
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</SX>
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[[Category: Ehrenberg, M.]]
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[[Category: Escherichia coli]]
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[[Category: Frank, J.]]
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[[Category: Large Structures]]
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[[Category: Harvey, S C.]]
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[[Category: Ehrenberg M]]
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[[Category: Li, W.]]
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[[Category: Frank J]]
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[[Category: Nielsen, R C.]]
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[[Category: Harvey SC]]
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[[Category: Nissen, P.]]
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[[Category: Li W]]
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[[Category: Sengupta, J.]]
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[[Category: Nielsen RC]]
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[[Category: Stagg, S M.]]
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[[Category: Nissen P]]
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[[Category: Valle, M.]]
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[[Category: Sengupta J]]
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[[Category: Zavialov, A.]]
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[[Category: Stagg SM]]
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[[Category: a-site trna]]
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[[Category: Valle M]]
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[[Category: Zavialov A]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:45:20 2008''
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Current revision

Coordinates of the A-site tRNA model fitted into the cryo-EM map of 70S ribosome in the pre-translocational state

1qzb, resolution 9.00Å

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