1r17

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[[Image:1r17.jpg|left|200px]]<br /><applet load="1r17" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1r17, resolution 1.86&Aring;" />
 
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'''Crystal Structure Analysis of S.epidermidis adhesin SdrG binding to Fibrinogen (adhesin-ligand complex)'''<br />
 
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==Overview==
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==Crystal Structure Analysis of S.epidermidis adhesin SdrG binding to Fibrinogen (adhesin-ligand complex)==
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Gram-positive pathogens such as staphylococci contain multiple cell wall-anchored proteins that serve as an interface between the microbe and its environment. Some of these proteins act as adhesins and mediate bacterial attachment to host tissues. SdrG is a cell wall-anchored adhesin from Staphylococcus epidermidis that binds to the Bbeta chain of human fibrinogen (Fg) and is necessary and sufficient for bacterial attachment to Fg-coated biomaterials. Here, we present the crystal structures of the ligand binding region of SdrG as an apoprotein and in complex with a synthetic peptide analogous to its binding site in Fg. Analysis of the crystal structures, along with mutational studies of both the protein and of the peptide, reveals that SdrG binds to its ligand with a dynamic "dock, lock, and latch" mechanism. We propose that this mechanism represents a general mode of ligand binding for structurally related cell wall-anchored proteins of gram-positive bacteria.
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<StructureSection load='1r17' size='340' side='right'caption='[[1r17]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1r17]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R17 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R17 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r17 OCA], [https://pdbe.org/1r17 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r17 RCSB], [https://www.ebi.ac.uk/pdbsum/1r17 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r17 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SDRG_STAEP SDRG_STAEP] Binds to the N-terminus of the B beta-chain of human fibrinogen and thereby interferes with thrombin cleavage and release of fibrinopeptide B, thus interfering with fibrin clot formation. This may hinder host defense against microbial infections.<ref>PMID:11371571</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r1/1r17_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r17 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1R17 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R17 OCA].
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*[[Fibrinogen binding protein|Fibrinogen binding protein]]
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== References ==
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==Reference==
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<references/>
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A "dock, lock, and latch" structural model for a staphylococcal adhesin binding to fibrinogen., Ponnuraj K, Bowden MG, Davis S, Gurusiddappa S, Moore D, Choe D, Xu Y, Hook M, Narayana SV, Cell. 2003 Oct 17;115(2):217-28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14567919 14567919]
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__TOC__
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[[Category: Single protein]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Staphylococcus epidermidis]]
[[Category: Staphylococcus epidermidis]]
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[[Category: Bowden, M G.]]
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[[Category: Bowden MG]]
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[[Category: Choe, D.]]
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[[Category: Choe D]]
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[[Category: Davis, S.]]
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[[Category: Davis S]]
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[[Category: Gurusiddappa, S.]]
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[[Category: Gurusiddappa S]]
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[[Category: Hook, M.]]
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[[Category: Hook M]]
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[[Category: Moore, D.]]
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[[Category: Moore D]]
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[[Category: Narayana, S V.L.]]
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[[Category: Narayana SVL]]
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[[Category: Ponnuraj, K.]]
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[[Category: Ponnuraj K]]
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[[Category: Xu, Y.]]
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[[Category: Xu Y]]
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[[Category: CA]]
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[[Category: mscramm-ligand complex]]
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[[Category: sdrg]]
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[[Category: sdrg-fibrinopeptide complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:45:56 2008''
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Current revision

Crystal Structure Analysis of S.epidermidis adhesin SdrG binding to Fibrinogen (adhesin-ligand complex)

PDB ID 1r17

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