1r20

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[[Image:1r20.gif|left|200px]]<br /><applet load="1r20" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1r20, resolution 3.0&Aring;" />
 
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'''Crystal structure of the ligand-binding domains of the heterodimer EcR/USP bound to the synthetic agonist BYI06830'''<br />
 
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==Overview==
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==Crystal structure of the ligand-binding domains of the heterodimer EcR/USP bound to the synthetic agonist BYI06830==
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The ecdysteroid hormones coordinate the major stages of insect development, notably moulting and metamorphosis, by binding to the ecdysone receptor (EcR); a ligand-inducible nuclear transcription factor. To bind either ligand or DNA, EcR must form a heterodimer with ultraspiracle (USP), the homologue of retinoid-X receptor. Here we report the crystal structures of the ligand-binding domains of the moth Heliothis virescens EcR-USP heterodimer in complex with the ecdysteroid ponasterone A and with a non-steroidal, lepidopteran-specific agonist BYI06830 used in agrochemical pest control. The two structures of EcR-USP emphasize the universality of heterodimerization as a general mechanism common to both vertebrates and invertebrates. Comparison of the EcR structures in complex with steroidal and non-steroidal ligands reveals radically different and only partially overlapping ligand-binding pockets that could not be predicted by molecular modelling and docking studies. These findings offer new perspectives for the design of insect-specific, environmentally safe insecticides. The concept of a ligand-dependent binding pocket in EcR provides an insight into the moulding of nuclear receptors to their ligand, and has potential applications for human nuclear receptors.
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<StructureSection load='1r20' size='340' side='right'caption='[[1r20]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1r20]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Heliothis_virescens Heliothis virescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R20 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R20 FirstGlance]. <br>
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1R20 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Heliothis_virescens Heliothis virescens] with <scene name='pdbligand=HWG:'>HWG</scene> and <scene name='pdbligand=EPH:'>EPH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R20 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPH:L-ALPHA-PHOSPHATIDYL-BETA-OLEOYL-GAMMA-PALMITOYL-PHOSPHATIDYLETHANOLAMINE'>EPH</scene>, <scene name='pdbligand=HWG:N-(TERT-BUTYL)-3,5-DIMETHYL-N-[(5-METHYL-2,3-DIHYDRO-1,4-BENZODIOXIN-6-YL)CARBONYL]BENZOHYDRAZIDE'>HWG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r20 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r20 OCA], [https://pdbe.org/1r20 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r20 RCSB], [https://www.ebi.ac.uk/pdbsum/1r20 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r20 ProSAT]</span></td></tr>
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Structural adaptability in the ligand-binding pocket of the ecdysone hormone receptor., Billas IM, Iwema T, Garnier JM, Mitschler A, Rochel N, Moras D, Nature. 2003 Nov 6;426(6962):91-6. Epub 2003 Nov 2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14595375 14595375]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q7SIF6_HELVI Q7SIF6_HELVI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r2/1r20_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r20 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Heliothis virescens]]
[[Category: Heliothis virescens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Billas, I M.L.]]
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[[Category: Billas IML]]
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[[Category: Garnier, J M.]]
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[[Category: Garnier JM]]
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[[Category: Iwema, T.]]
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[[Category: Iwema T]]
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[[Category: Mitschler, A.]]
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[[Category: Mitschler A]]
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[[Category: Moras, D.]]
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[[Category: Moras D]]
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[[Category: Rochel, N.]]
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[[Category: Rochel N]]
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[[Category: SPINE, Structural Proteomics in Europe.]]
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[[Category: EPH]]
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[[Category: HWG]]
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[[Category: alpha-helical sandwich]]
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[[Category: heterodimer]]
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[[Category: nuclear receptor]]
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[[Category: spine]]
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[[Category: structural genomics]]
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[[Category: structural proteomics in europe]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:46:09 2008''
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Current revision

Crystal structure of the ligand-binding domains of the heterodimer EcR/USP bound to the synthetic agonist BYI06830

PDB ID 1r20

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