1r27

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[[Image:1r27.gif|left|200px]]<br /><applet load="1r27" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1r27, resolution 2.00&Aring;" />
 
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'''Crystal Structure of NarGH complex'''<br />
 
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==Overview==
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==Crystal Structure of NarGH complex==
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The structure of the catalytic and electron-transfer subunits (NarGH) of the integral membrane protein, respiratory nitrate reductase (Nar) has been determined to 2.0 A resolution revealing the molecular architecture of this Mo-bisMGD (molybdopterin-guanine-dinucleotide) containing enzyme which includes a previously undetected FeS cluster. Nar, together with the related enzyme formate dehydrogenase (Fdh-N), is a key enzyme in the generation of proton motive force across the membrane in Escherichia coli nitrate respiration. A comparative study revealed that Nar and Fdh-N employ different approaches for acquiring substrate, reflecting different catalytic mechanisms. Nar uses a very narrow and nonpolar substrate-conducting cavity with a nonspecific substrate binding site, whereas Fdh-N accommodates a wider, positively charged substrate-conducting cavity with a more specific substrate binding site. The Nar structure also demonstrates the first example of an Asp side chain acting as a Mo ligand providing a structural basis for the classification of Mo-bisMGD enzymes.
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<StructureSection load='1r27' size='340' side='right'caption='[[1r27]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1r27]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R27 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=MO:MOLYBDENUM+ATOM'>MO</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r27 OCA], [https://pdbe.org/1r27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r27 RCSB], [https://www.ebi.ac.uk/pdbsum/1r27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r27 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NARG_ECOLI NARG_ECOLI] The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The alpha chain is the actual site of nitrate reduction.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r2/1r27_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r27 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1R27 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MO:'>MO</scene>, <scene name='pdbligand=F3S:'>F3S</scene>, <scene name='pdbligand=SF4:'>SF4</scene> and <scene name='pdbligand=MGD:'>MGD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrate_reductase Nitrate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.4 1.7.99.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R27 OCA].
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*[[Nitrate reductase|Nitrate reductase]]
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__TOC__
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==Reference==
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</StructureSection>
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Architecture of NarGH reveals a structural classification of Mo-bisMGD enzymes., Jormakka M, Richardson D, Byrne B, Iwata S, Structure. 2004 Jan;12(1):95-104. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14725769 14725769]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Nitrate reductase]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Byrne B]]
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[[Category: Byrne, B.]]
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[[Category: Iwata S]]
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[[Category: Iwata, S.]]
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[[Category: Jormakka M]]
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[[Category: Jormakka, M.]]
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[[Category: Richardson D]]
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[[Category: Richardson, D.]]
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[[Category: F3S]]
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[[Category: MGD]]
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[[Category: MO]]
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[[Category: SF4]]
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[[Category: beta barrel; x-ray crystallography]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:46:14 2008''
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Current revision

Crystal Structure of NarGH complex

PDB ID 1r27

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