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1r3o

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[[Image:1r3o.gif|left|200px]]<br /><applet load="1r3o" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1r3o, resolution 1.90&Aring;" />
 
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'''Crystal structure of the first RNA duplex in L-conformation at 1.9A resolution'''<br />
 
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==Overview==
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==Crystal structure of the first RNA duplex in L-conformation at 1.9A resolution==
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Nucleic acid molecules in the mirror image or L-configuration are unknown in nature and are extraordinarily resistant to biological degradation. The identification of functional L-oligonucleotides called Spiegelmers offers a novel approach for drug discovery based on RNA. The sequence r(CUGGGCGG).r(CCGCCUGG) was chosen as a model system for structural analysis of helices in the L-configuration as the structure of the D-form of this sequence has previously been determined in structural studies of 5S RNA domains, in particular domain E of the Thermus flavus 5S rRNA [Perbandt et al. (2001), Acta Cryst. D57, 219-224]. Unexpectedly, the results of crystallization trials showed little similarity between the D- and the L-forms of the duplex in either the crystallization hits or the diffraction performance. The crystal structure of this L-RNA duplex has been determined at 1.9 A resolution with R(work) and R(free) of 23.8 and 28.6%, respectively. The crystals belong to space group R32, with unit-cell parameters a = 45.7, c = 264.6 A. Although there are two molecules in the asymmetric unit rather than one, the structure of the L-form arranges helical pairs in a head-to-tail fashion to form pseudo-continuous infinite helices in the crystal as in the D-form. On the other hand, the wobble-like G.C(+) base pair seen in the D-RNA analogue does not appear in the L-RNA duplex, which forms a regular double-helical structure with typical Watson-Crick base pairing.
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<StructureSection load='1r3o' size='340' side='right'caption='[[1r3o]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1r3o]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R3O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R3O FirstGlance]. <br>
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1R3O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R3O OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0C:L-CYTIDINE-5-MONOPHOSPHATE'>0C</scene>, <scene name='pdbligand=0G:L-GUANOSINE-5-MONOPHOSPHATE'>0G</scene>, <scene name='pdbligand=0U:L-URIDINE-5-MONOPHOSPHATE'>0U</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r3o OCA], [https://pdbe.org/1r3o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r3o RCSB], [https://www.ebi.ac.uk/pdbsum/1r3o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r3o ProSAT]</span></td></tr>
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First look at RNA in L-configuration., Vallazza M, Perbandt M, Klussmann S, Rypniewski W, Einspahr HM, Erdmann VA, Betzel Ch, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):1-7. Epub 2003 Dec, 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14684885 14684885]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Betzel, C.]]
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</StructureSection>
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[[Category: Erdmann, V A.]]
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[[Category: Large Structures]]
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[[Category: Klussmann, S.]]
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[[Category: Betzel C]]
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[[Category: Perbandt, M.]]
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[[Category: Erdmann VA]]
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[[Category: Rypniewski, W.]]
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[[Category: Klussmann S]]
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[[Category: Vallazza, M.]]
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[[Category: Perbandt M]]
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[[Category: l-rna]]
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[[Category: Rypniewski W]]
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[[Category: Vallazza M]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:46:48 2008''
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Current revision

Crystal structure of the first RNA duplex in L-conformation at 1.9A resolution

PDB ID 1r3o

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