1ire

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[[Image:1ire.png|left|200px]]
 
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{{STRUCTURE_1ire| PDB=1ire | SCENE= }}
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==Crystal Structure of Co-type nitrile hydratase from Pseudonocardia thermophila==
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<StructureSection load='1ire' size='340' side='right'caption='[[1ire]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ire]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudonocardia_thermophila Pseudonocardia thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IRE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IRE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ire FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ire OCA], [https://pdbe.org/1ire PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ire RCSB], [https://www.ebi.ac.uk/pdbsum/1ire PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ire ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NHAA_PSETH NHAA_PSETH] NHase catalyzes the hydration of various nitrile compounds to the corresponding amides.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ir/1ire_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ire ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of cobalt-containing nitrile hydratase from Pseudonocardia thermophila JCM 3095 at 1.8 A resolution revealed the structure of the noncorrin cobalt at the catalytic center. Two cysteine residues (alphaCys(111) and alphaCys(113)) coordinated to the cobalt were posttranslationally modified to cysteine-sulfinic acid and to cysteine-sulfenic acid, respectively, like in iron-containing nitrile hydratase. A tryptophan residue (betaTrp(72)), which may be involved in substrate binding, replaced the tyrosine residue of iron-containing nitrile hydratase. The difference seems to be responsible for the preference for aromatic nitriles rather than aliphatic ones of cobalt-containing nitrile hydratase.
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===Crystal Structure of Co-type nitrile hydratase from Pseudonocardia thermophila===
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Crystal structure of cobalt-containing nitrile hydratase.,Miyanaga A, Fushinobu S, Ito K, Wakagi T Biochem Biophys Res Commun. 2001 Nov 16;288(5):1169-74. PMID:11700034<ref>PMID:11700034</ref>
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{{ABSTRACT_PUBMED_11700034}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1ire" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[1ire]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudonocardia_thermophila Pseudonocardia thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IRE OCA].
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*[[Nitrile hydratase|Nitrile hydratase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:011700034</ref><references group="xtra"/>
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__TOC__
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[[Category: Nitrile hydratase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudonocardia thermophila]]
[[Category: Pseudonocardia thermophila]]
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[[Category: Fushinobu, S.]]
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[[Category: Fushinobu S]]
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[[Category: Ito, K.]]
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[[Category: Ito K]]
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[[Category: Miyanaga, A.]]
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[[Category: Miyanaga A]]
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[[Category: Wakagi, T.]]
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[[Category: Wakagi T]]
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[[Category: Cysteine-sulfenic acid]]
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[[Category: Cysteine-sulfinic acid]]
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[[Category: Hydration]]
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[[Category: Lyase]]
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[[Category: Nitrile]]
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[[Category: Non-corrin cobalt]]
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[[Category: Post-translational modification]]
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Current revision

Crystal Structure of Co-type nitrile hydratase from Pseudonocardia thermophila

PDB ID 1ire

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