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1r5m

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[[Image:1r5m.gif|left|200px]]<br /><applet load="1r5m" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1r5m, resolution 1.55&Aring;" />
 
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'''Crystal Structure Of The C-Terminal WD40 Domain Of Sif2'''<br />
 
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==Overview==
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==Crystal Structure Of The C-Terminal WD40 Domain Of Sif2==
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In Saccharomyces cerevisiae, the SIF2 gene product is an integral component of the Set3 complex (SET3C), an assembly of proteins with some homology to the human SMRT and N-CoR corepressor complexes. SET3C has histone deacetylase activity that is responsible for repressing a set of meiotic genes. We have determined the X-ray crystal structure of a 46 kDa C-terminal domain of a SET3C core protein, Sif2p to 1.55 A resolution and a crystallographic R-factor of 19.0%. This domain contains an unusual eight-bladed beta-propeller structure, which differs from other transcriptional corepressor structures such as yeast Tup1p and human groucho (Gro)/TLE1, which have only seven. We have demonstrated intact Sif2p is a tetramer and the N-terminal LisH (Lis-homology)-containing domain mediates tetramerization and interaction with another component of SET3C, Snt1p. Multiple sequence alignments indicate that a surface on the "top" of the protein is conserved among species, suggesting that it may play a common role in binding partner proteins. Since Sif2p appears to be the yeast homolog of human TBL1 and TBLR1, which function in the N-CoR/SMRT complexes, its structural and oligomeric properties are likely to be very similar.
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<StructureSection load='1r5m' size='340' side='right'caption='[[1r5m]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1r5m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R5M FirstGlance]. <br>
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1R5M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5M OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r5m OCA], [https://pdbe.org/1r5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r5m RCSB], [https://www.ebi.ac.uk/pdbsum/1r5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r5m ProSAT]</span></td></tr>
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The structure of Sif2p, a WD repeat protein functioning in the SET3 corepressor complex., Cerna D, Wilson DK, J Mol Biol. 2005 Aug 26;351(4):923-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16051270 16051270]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SIF2_YEAST SIF2_YEAST] Antagonizes telomeric silencing in yeast. May recruit SIR4 to non-telomeric sites or repression.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r5/1r5m_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r5m ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Cerna D]]
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[[Category: Cerna, D.]]
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[[Category: Wilson DK]]
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[[Category: Wilson, D K.]]
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[[Category: SO4]]
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[[Category: beta propeller]]
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[[Category: transcription corepressor]]
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[[Category: wd40 repeat]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:47:18 2008''
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Current revision

Crystal Structure Of The C-Terminal WD40 Domain Of Sif2

PDB ID 1r5m

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