1r62

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[[Image:1r62.jpg|left|200px]]<br /><applet load="1r62" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1r62, resolution 1.60&Aring;" />
 
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'''Crystal structure of the C-terminal Domain of the Two-Component System Transmitter Protein NRII (NtrB)'''<br />
 
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==Overview==
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==Crystal structure of the C-terminal Domain of the Two-Component System Transmitter Protein NRII (NtrB)==
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The kinase/phosphatase nitrogen regulator II (NRII, NtrB) is a member of the transmitter protein family of conserved two-component signal transduction systems. The kinase activity of NRII brings about the phosphorylation of the transcription factor nitrogen regulator I (NRI, NtrC), causing the activation of Ntr gene transcription. The phosphatase activity of NRII results in the inactivation of NRI-P. The activities of NRII are regulated by the signal transduction protein encoded by glnB, PII protein, which upon binding to NRII inhibits the kinase and activates the phosphatase activity. The C-terminal ATP-binding domain of NRII is required for both the kinase and phosphatase activities and contains the PII binding site. Here, we present the crystal structure of the C-terminal domain of a mutant form of NRII, NRII-Y302N, at 1.6 A resolution and compare this structure to the analogous domains of other two-component system transmitter proteins. While the C-terminal domain of NRII shares the general tertiary structure seen in CheA, PhoQ, and EnvZ transmitter proteins, it contains a distinct beta-hairpin projection that is absent in these related proteins. This projection is near the site of a well-characterized mutation that reduces the binding of PII and near other less-characterized mutations that affect the phosphatase activity of NRII. Sequence alignment suggests that the beta-hairpin projection is present in NRII proteins from various organisms, and absent in other transmitter proteins from Escherichia coliK-12. This unique structural element in the NRII C-terminal domain may play a role in binding PII or in intramolecular signal transduction.
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<StructureSection load='1r62' size='340' side='right'caption='[[1r62]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1r62]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R62 FirstGlance]. <br>
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1R62 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R62 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r62 OCA], [https://pdbe.org/1r62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r62 RCSB], [https://www.ebi.ac.uk/pdbsum/1r62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r62 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of the C-terminal domain of the two-component system transmitter protein nitrogen regulator II (NRII; NtrB), regulator of nitrogen assimilation in Escherichia coli., Song Y, Peisach D, Pioszak AA, Xu Z, Ninfa AJ, Biochemistry. 2004 Jun 1;43(21):6670-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15157101 15157101]
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== Function ==
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[[Category: Escherichia coli]]
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[https://www.uniprot.org/uniprot/NTRB_ECOLI NTRB_ECOLI] NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of NtrC. In nitrogen limitation NtrB activates NtrC by phosphorylating it, while in nitrogen excess NtrC is dephosphorylated and consequently inactivated by NtrB.
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Ninfa, A J.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Peisach, D.]]
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Check<jmol>
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[[Category: Pioszak, A A.]]
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<jmolCheckbox>
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[[Category: Song, Y.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r6/1r62_consurf.spt"</scriptWhenChecked>
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[[Category: Xu, Z.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: histidine kinase]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: nrii]]
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</jmolCheckbox>
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[[Category: pii]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r62 ConSurf].
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[[Category: two component system]]
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<div style="clear:both"></div>
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__TOC__
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:47:28 2008''
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</StructureSection>
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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[[Category: Ninfa AJ]]
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[[Category: Peisach D]]
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[[Category: Pioszak AA]]
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[[Category: Song Y]]
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[[Category: Xu Z]]

Current revision

Crystal structure of the C-terminal Domain of the Two-Component System Transmitter Protein NRII (NtrB)

PDB ID 1r62

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