1dtn

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[[Image:1dtn.gif|left|200px]]<br />
 
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<applet load="1dtn" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1dtn, resolution 2.1&Aring;" />
 
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'''MANDELATE RACEMASE MUTANT D270N CO-CRYSTALLIZED WITH (S)-ATROLACTATE'''<br />
 
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==Overview==
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==MANDELATE RACEMASE MUTANT D270N CO-CRYSTALLIZED WITH (S)-ATROLACTATE==
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In the high-resolution X-ray structure of mandelate racemase (MR) with the, competitive inhibitor (S)-atrolactate bound in the active site [Landro, J., A., Gerlt, J. A., Kozarich, J. W., Koo, C. W., Shah, V. J., Kenyon, G. L., Neidhart, D. J., Fujita, J., &amp; Petsko, G. A. (1994) Biochemistry 33, 635-643], the carboxylic acid group of Glu 317 is hydrogen-bonded to the, carboxylate group of the bound inhibitor. This geometry suggests that the, carboxylic acid functional group of Glu 317 participates as a general acid, catalyst in the concerted general acid-general base catalyzed formation of, a stabilized enolic tautomer of mandelic acid as a reaction intermediate., To test this hypothesis, the E317Q mutant of MR was constructed and, subjected to high-resolution X-ray structural analysis ... [[http://ispc.weizmann.ac.il/pmbin/getpm?7893689 (full description)]]
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<StructureSection load='1dtn' size='340' side='right'caption='[[1dtn]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1dtn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DTN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APG:ATROLACTIC+ACID+(2-PHENYL-LACTIC+ACID)'>APG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dtn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dtn OCA], [https://pdbe.org/1dtn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dtn RCSB], [https://www.ebi.ac.uk/pdbsum/1dtn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dtn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MANR_PSEPU MANR_PSEPU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dt/1dtn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dtn ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1DTN is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with MG and APG as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Mandelate_racemase Mandelate racemase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.2.2 5.1.2.2]]. Structure known Active Sites: ACT, CAR and MTL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DTN OCA]].
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*[[Mandelate racemase|Mandelate racemase]]
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__TOC__
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==Reference==
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</StructureSection>
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Mechanism of the reaction catalyzed by mandelate racemase: importance of electrophilic catalysis by glutamic acid 317., Mitra B, Kallarakal AT, Kozarich JW, Gerlt JA, Clifton JG, Petsko GA, Kenyon GL, Biochemistry. 1995 Mar 7;34(9):2777-87. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7893689 7893689]
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[[Category: Large Structures]]
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[[Category: Mandelate racemase]]
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[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Clifton JG]]
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[[Category: Clifton, J.G.]]
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[[Category: Petsko GA]]
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[[Category: Petsko, G.A.]]
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[[Category: APG]]
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[[Category: MG]]
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[[Category: isomerase]]
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[[Category: mandelate pathway]]
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[[Category: racemase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:01:45 2007''
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Current revision

MANDELATE RACEMASE MUTANT D270N CO-CRYSTALLIZED WITH (S)-ATROLACTATE

PDB ID 1dtn

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