1r8o

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:18, 30 October 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1r8o.jpg|left|200px]]<br /><applet load="1r8o" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1r8o, resolution 1.83&Aring;" />
 
-
'''Crystal structure of an unusual Kunitz-type trypsin inhibitor from Copaifera langsdorffii seeds'''<br />
 
-
==Overview==
+
==Crystal structure of an unusual Kunitz-type trypsin inhibitor from Copaifera langsdorffii seeds==
 +
<StructureSection load='1r8o' size='340' side='right'caption='[[1r8o]], [[Resolution|resolution]] 1.83&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1r8o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Copaifera_langsdorffii Copaifera langsdorffii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R8O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R8O FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.83&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r8o OCA], [https://pdbe.org/1r8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r8o RCSB], [https://www.ebi.ac.uk/pdbsum/1r8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r8o ProSAT]</span></td></tr>
 +
</table>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r8/1r8o_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r8o ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffii is reported. The structure was solved by MIRAS procedure and refined to a crystallographic residual of 17.3% (R(free) = 20.3%) at 1.8 A resolution. Two isomorphous derivatives were obtained by quick cryo-soaking approach. CTI is the first structure of a member of Kunitz (STI) family formed by two noncovalently bound polypeptide chains and only one disulfide bridge. A standard Kunitz-type inhibitor has a single polypeptide chain and two disulfide bridges. Structural features granting CTI high inhibitory activity are discussed.
The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffii is reported. The structure was solved by MIRAS procedure and refined to a crystallographic residual of 17.3% (R(free) = 20.3%) at 1.8 A resolution. Two isomorphous derivatives were obtained by quick cryo-soaking approach. CTI is the first structure of a member of Kunitz (STI) family formed by two noncovalently bound polypeptide chains and only one disulfide bridge. A standard Kunitz-type inhibitor has a single polypeptide chain and two disulfide bridges. Structural features granting CTI high inhibitory activity are discussed.
-
==About this Structure==
+
Three-dimensional structure of an unusual Kunitz (STI) type trypsin inhibitor from Copaifera langsdorffii.,Krauchenco S, Nagem RA, da Silva JA, Marangoni S, Polikarpov I Biochimie. 2004 Mar;86(3):167-72. PMID:15134830<ref>PMID:15134830</ref>
-
1R8O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Copaifera_langsdorffii Copaifera langsdorffii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R8O OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Three-dimensional structure of an unusual Kunitz (STI) type trypsin inhibitor from Copaifera langsdorffii., Krauchenco S, Nagem RA, da Silva JA, Marangoni S, Polikarpov I, Biochimie. 2004 Mar;86(3):167-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15134830 15134830]
+
</div>
-
[[Category: Copaifera langsdorffii]]
+
<div class="pdbe-citations 1r8o" style="background-color:#fffaf0;"></div>
-
[[Category: Protein complex]]
+
-
[[Category: Krauchenco, S.]]
+
-
[[Category: Marangoni, S.]]
+
-
[[Category: Nagem, R A.P.]]
+
-
[[Category: Polikarpov, I.]]
+
-
[[Category: Silva, J A.da.]]
+
-
[[Category: beta-trefoil fold]]
+
-
[[Category: copaifera langsdorffii]]
+
-
[[Category: kunitz (sti) trypsin inhibitor]]
+
-
[[Category: x-ray structure]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:48:18 2008''
+
==See Also==
 +
*[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Copaifera langsdorffii]]
 +
[[Category: Large Structures]]
 +
[[Category: Krauchenco S]]
 +
[[Category: Marangoni S]]
 +
[[Category: Nagem RAP]]
 +
[[Category: Polikarpov I]]
 +
[[Category: Da Silva JA]]

Current revision

Crystal structure of an unusual Kunitz-type trypsin inhibitor from Copaifera langsdorffii seeds

PDB ID 1r8o

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools