1r8o
From Proteopedia
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| - | [[Image:1r8o.jpg|left|200px]]<br /><applet load="1r8o" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1r8o, resolution 1.83Å" /> | ||
| - | '''Crystal structure of an unusual Kunitz-type trypsin inhibitor from Copaifera langsdorffii seeds'''<br /> | ||
| - | == | + | ==Crystal structure of an unusual Kunitz-type trypsin inhibitor from Copaifera langsdorffii seeds== |
| + | <StructureSection load='1r8o' size='340' side='right'caption='[[1r8o]], [[Resolution|resolution]] 1.83Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1r8o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Copaifera_langsdorffii Copaifera langsdorffii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R8O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R8O FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.83Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r8o OCA], [https://pdbe.org/1r8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r8o RCSB], [https://www.ebi.ac.uk/pdbsum/1r8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r8o ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r8/1r8o_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r8o ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffii is reported. The structure was solved by MIRAS procedure and refined to a crystallographic residual of 17.3% (R(free) = 20.3%) at 1.8 A resolution. Two isomorphous derivatives were obtained by quick cryo-soaking approach. CTI is the first structure of a member of Kunitz (STI) family formed by two noncovalently bound polypeptide chains and only one disulfide bridge. A standard Kunitz-type inhibitor has a single polypeptide chain and two disulfide bridges. Structural features granting CTI high inhibitory activity are discussed. | The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffii is reported. The structure was solved by MIRAS procedure and refined to a crystallographic residual of 17.3% (R(free) = 20.3%) at 1.8 A resolution. Two isomorphous derivatives were obtained by quick cryo-soaking approach. CTI is the first structure of a member of Kunitz (STI) family formed by two noncovalently bound polypeptide chains and only one disulfide bridge. A standard Kunitz-type inhibitor has a single polypeptide chain and two disulfide bridges. Structural features granting CTI high inhibitory activity are discussed. | ||
| - | + | Three-dimensional structure of an unusual Kunitz (STI) type trypsin inhibitor from Copaifera langsdorffii.,Krauchenco S, Nagem RA, da Silva JA, Marangoni S, Polikarpov I Biochimie. 2004 Mar;86(3):167-72. PMID:15134830<ref>PMID:15134830</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1r8o" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Copaifera langsdorffii]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Krauchenco S]] | ||
| + | [[Category: Marangoni S]] | ||
| + | [[Category: Nagem RAP]] | ||
| + | [[Category: Polikarpov I]] | ||
| + | [[Category: Da Silva JA]] | ||
Current revision
Crystal structure of an unusual Kunitz-type trypsin inhibitor from Copaifera langsdorffii seeds
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