1rb4

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[[Image:1rb4.jpg|left|200px]]<br /><applet load="1rb4" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rb4, resolution 1.9&Aring;" />
 
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'''ANTIPARALLEL TRIMER OF GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A TETRAGONAL AUTOMATIC SOLUTION'''<br />
 
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==Overview==
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==ANTIPARALLEL TRIMER OF GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A TETRAGONAL AUTOMATIC SOLUTION==
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Efficient determination of protein crystal structures requires automated x-ray data analysis. Here, we describe the expert system ELVES and its use to determine automatically the structure of a 12-kDa protein. Multiwavelength anomalous diffraction analysis of a selenomethionyl derivative was used to image the Asn-16-Ala variant of the GCN4 leucine zipper. In contrast to the parallel, dimeric coiled coil formed by the WT sequence, the mutant unexpectedly formed an antiparallel trimer. This structural switch reveals how avoidance of core cavities at a single site can select the native fold of a protein. All structure calculations, including indexing, data processing, locating heavy atoms, phasing by multiwavelength anomalous diffraction, model building, and refinement, were completed without human intervention. The results demonstrate the feasibility of automated methods for determining high-resolution, x-ray crystal structures of proteins.
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<StructureSection load='1rb4' size='340' side='right'caption='[[1rb4]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rb4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RB4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rb4 OCA], [https://pdbe.org/1rb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rb4 RCSB], [https://www.ebi.ac.uk/pdbsum/1rb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rb4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.
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==About this Structure==
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==See Also==
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1RB4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RB4 OCA].
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*[[Gcn4 3D Structures|Gcn4 3D Structures]]
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*[[Gnc4 3D Structures|Gnc4 3D Structures]]
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==Reference==
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__TOC__
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Automated protein crystal structure determination using ELVES., Holton J, Alber T, Proc Natl Acad Sci U S A. 2004 Feb 10;101(6):1537-42. Epub 2004 Jan 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14752198 14752198]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Alber, T.]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Holton, J.]]
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[[Category: Alber T]]
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[[Category: automation]]
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[[Category: Holton J]]
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[[Category: coiled coil]]
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[[Category: leucine zipper]]
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[[Category: peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:48:59 2008''
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Current revision

ANTIPARALLEL TRIMER OF GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A TETRAGONAL AUTOMATIC SOLUTION

PDB ID 1rb4

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