1ri2

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[[Image:1ri2.gif|left|200px]]<br /><applet load="1ri2" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ri2, resolution 2.70&Aring;" />
 
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'''Structure and mechanism of mRNA cap (guanine N-7) methyltransferase'''<br />
 
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==Overview==
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==Structure and mechanism of mRNA cap (guanine N-7) methyltransferase==
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A suite of crystal structures is reported for a cellular mRNA cap (guanine-N7) methyltransferase in complex with AdoMet, AdoHcy, and the cap guanylate. Superposition of ligand complexes suggests an in-line mechanism of methyl transfer, albeit without direct contacts between the enzyme and either the N7 atom of guanine (the attacking nucleophile), the methyl carbon of AdoMet, or the sulfur of AdoMet/AdoHcy (the leaving group). The structures indicate that catalysis of cap N7 methylation is accomplished by optimizing proximity and orientation of the substrates, assisted by a favorable electrostatic environment. The enzyme-ligand structures, together with new mutational data, fully account for the biochemical specificity of the cap guanine-N7 methylation reaction, an essential and defining step of eukaryotic mRNA synthesis.
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<StructureSection load='1ri2' size='340' side='right'caption='[[1ri2]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ri2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RI2 FirstGlance]. <br>
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1RI2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi] with <scene name='pdbligand=GTG:'>GTG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI2 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ri2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri2 OCA], [https://pdbe.org/1ri2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ri2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ri2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ri2 ProSAT]</span></td></tr>
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Structure and mechanism of mRNA cap (guanine-N7) methyltransferase., Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD, Mol Cell. 2004 Jan 16;13(1):77-89. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14731396 14731396]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MCES_ENCCU MCES_ENCCU] mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.<ref>PMID:15760890</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ri/1ri2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ri2 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Encephalitozoon cuniculi]]
[[Category: Encephalitozoon cuniculi]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fabrega, C.]]
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[[Category: Fabrega C]]
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[[Category: Hausmann, S.]]
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[[Category: Hausmann S]]
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[[Category: Lima, C D.]]
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[[Category: Lima CD]]
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[[Category: Shen, V.]]
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[[Category: Shen V]]
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[[Category: Shuman, S.]]
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[[Category: Shuman S]]
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[[Category: GTG]]
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[[Category: cap]]
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[[Category: m7g]]
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[[Category: messenger rna cap]]
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[[Category: methyltransferase]]
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[[Category: rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:51:09 2008''
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Current revision

Structure and mechanism of mRNA cap (guanine N-7) methyltransferase

PDB ID 1ri2

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