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1jwg

From Proteopedia

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[[Image:1jwg.png|left|200px]]
 
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{{STRUCTURE_1jwg| PDB=1jwg | SCENE= }}
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==VHS Domain of human GGA1 complexed with cation-independent M6PR C-terminal Peptide==
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<StructureSection load='1jwg' size='340' side='right'caption='[[1jwg]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jwg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JWG FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwg OCA], [https://pdbe.org/1jwg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jwg RCSB], [https://www.ebi.ac.uk/pdbsum/1jwg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jwg ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GGA1_HUMAN GGA1_HUMAN] Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.<ref>PMID:11301005</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jw/1jwg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jwg ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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GGAs (Golgi-localizing, gamma-adaptin ear homology domain, ARF-interacting proteins) are critical for the transport of soluble proteins from the trans-Golgi network (TGN) to endosomes/lysosomes by means of interactions with TGN-sorting receptors, ADP-ribosylation factor (ARF), and clathrin. The amino-terminal VHS domains of GGAs form complexes with the cytoplasmic domains of sorting receptors by recognizing acidic-cluster dileucine (ACLL) sequences. Here we report the X-ray structure of the GGA1 VHS domain alone, and in complex with the carboxy-terminal peptide of cation-independent mannose 6-phosphate receptor containing an ACLL sequence. The VHS domain forms a super helix with eight alpha-helices, similar to the VHS domains of TOM1 and Hrs. Unidirectional movements of helices alpha6 and alpha8, and some of their side chains, create a set of electrostatic and hydrophobic interactions for correct recognition of the ACLL peptide. This recognition mechanism provides the basis for regulation of protein transport from the TGN to endosomes/lysosomes, which is shared by sortilin and low-density lipoprotein receptor-related protein.
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===VHS Domain of human GGA1 complexed with cation-independent M6PR C-terminal Peptide===
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Structural basis for recognition of acidic-cluster dileucine sequence by GGA1.,Shiba T, Takatsu H, Nogi T, Matsugaki N, Kawasaki M, Igarashi N, Suzuki M, Kato R, Earnest T, Nakayama K, Wakatsuki S Nature. 2002 Feb 21;415(6874):937-41. PMID:11859376<ref>PMID:11859376</ref>
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{{ABSTRACT_PUBMED_11859376}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1jwg" style="background-color:#fffaf0;"></div>
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[[1jwg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWG OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:011859376</ref><references group="xtra"/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Earnest, T.]]
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[[Category: Large Structures]]
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[[Category: Igarashi, N.]]
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[[Category: Earnest T]]
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[[Category: Kato, R.]]
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[[Category: Igarashi N]]
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[[Category: Kawasaki, M.]]
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[[Category: Kato R]]
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[[Category: Matsugaki, N.]]
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[[Category: Kawasaki M]]
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[[Category: Nakayama, K.]]
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[[Category: Matsugaki N]]
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[[Category: Nogi, T.]]
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[[Category: Nakayama K]]
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[[Category: Shiba, T.]]
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[[Category: Nogi T]]
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[[Category: Suzuki, M.]]
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[[Category: Shiba T]]
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[[Category: Takatsu, H.]]
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[[Category: Suzuki M]]
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[[Category: Wakatsuki, S.]]
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[[Category: Takatsu H]]
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[[Category: Protein transport-protein binding complex]]
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[[Category: Wakatsuki S]]
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[[Category: Protein-peptide complex]]
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[[Category: Super helix]]
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Current revision

VHS Domain of human GGA1 complexed with cation-independent M6PR C-terminal Peptide

PDB ID 1jwg

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