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1k5w

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[[Image:1k5w.png|left|200px]]
 
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{{STRUCTURE_1k5w| PDB=1k5w | SCENE= }}
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==THREE-DIMENSIONAL STRUCTURE OF THE SYNAPTOTAGMIN 1 C2B-DOMAIN: SYNAPTOTAGMIN 1 AS A PHOSPHOLIPID BINDING MACHINE==
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<StructureSection load='1k5w' size='340' side='right'caption='[[1k5w]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1k5w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K5W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K5W FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k5w OCA], [https://pdbe.org/1k5w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k5w RCSB], [https://www.ebi.ac.uk/pdbsum/1k5w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k5w ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SYT1_RAT SYT1_RAT] May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k5/1k5w_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k5w ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Synaptotagmin 1 probably functions as a Ca2+ sensor in neurotransmitter release via its two C2-domains, but no common Ca2+-dependent activity that could underlie a cooperative action between them has been described. The NMR structure of the C2B-domain now reveals a beta sandwich that exhibits striking similarities and differences with the C2A-domain. Whereas the bottom face of the C2B-domain has two additional alpha helices that may be involved in specialized Ca2+-independent functions, the top face binds two Ca2+ ions and is remarkably similar to the C2A-domain. Consistent with these results, but in contrast to previous studies, we find that the C2B-domain binds phospholipids in a Ca2+-dependent manner similarly to the C2A-domain. These results suggest a novel view of synaptotagmin function whereby the two C2-domains cooperate in a common activity, Ca2+-dependent phospholipid binding, to trigger neurotransmitter release.
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===THREE-DIMENSIONAL STRUCTURE OF THE SYNAPTOTAGMIN 1 C2B-DOMAIN: SYNAPTOTAGMIN 1 AS A PHOSPHOLIPID BINDING MACHINE===
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Three-dimensional structure of the synaptotagmin 1 C2B-domain: synaptotagmin 1 as a phospholipid binding machine.,Fernandez I, Arac D, Ubach J, Gerber SH, Shin O, Gao Y, Anderson RG, Sudhof TC, Rizo J Neuron. 2001 Dec 20;32(6):1057-69. PMID:11754837<ref>PMID:11754837</ref>
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{{ABSTRACT_PUBMED_11754837}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1k5w" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[1k5w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K5W OCA].
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*[[Synaptotagmin 3D structures|Synaptotagmin 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:011754837</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Anderson, R G.W.]]
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[[Category: Anderson RGW]]
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[[Category: Arac, D.]]
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[[Category: Arac D]]
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[[Category: Fernandez, I.]]
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[[Category: Fernandez I]]
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[[Category: Gao, Y.]]
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[[Category: Gao Y]]
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[[Category: Gerber, S H.]]
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[[Category: Gerber SH]]
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[[Category: Rizo, J.]]
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[[Category: Rizo J]]
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[[Category: Shin, O.]]
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[[Category: Shin O]]
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[[Category: Sudhof, T C.]]
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[[Category: Sudhof TC]]
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[[Category: Ubach, J.]]
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[[Category: Ubach J]]
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[[Category: C2-domain]]
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[[Category: C2b-domain]]
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[[Category: Calcium-binding]]
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[[Category: Endocytosis-exocytosis complex]]
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[[Category: Neurotransmitter release]]
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[[Category: Phospholipid-binding]]
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[[Category: Synapsis]]
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[[Category: Synaptic vesicle exocytosis]]
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[[Category: Synaptotagmin i]]
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Current revision

THREE-DIMENSIONAL STRUCTURE OF THE SYNAPTOTAGMIN 1 C2B-DOMAIN: SYNAPTOTAGMIN 1 AS A PHOSPHOLIPID BINDING MACHINE

PDB ID 1k5w

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