1o0p

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[[Image:1o0p.png|left|200px]]
 
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{{STRUCTURE_1o0p| PDB=1o0p | SCENE= }}
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==Solution Structure of the third RNA Recognition Motif (RRM) of U2AF65 in complex with an N-terminal SF1 peptide==
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<StructureSection load='1o0p' size='340' side='right'caption='[[1o0p]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1o0p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O0P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O0P FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o0p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o0p OCA], [https://pdbe.org/1o0p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o0p RCSB], [https://www.ebi.ac.uk/pdbsum/1o0p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o0p ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/U2AF2_HUMAN U2AF2_HUMAN] Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10.<ref>PMID:15009664</ref> <ref>PMID:19470458</ref> <ref>PMID:19574390</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o0/1o0p_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o0p ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The essential splicing factors SF1 and U2AF play an important role in the recognition of the pre-mRNA 3' splice site during early spliceosome assembly. The structure of the C-terminal RRM (RRM3) of human U2AF(65) complexed to an N-terminal peptide of SF1 reveals an extended negatively charged helix A and an additional helix C. Helix C shields the potential RNA binding surface. SF1 binds to the opposite, helical face of RRM3. It inserts a conserved tryptophan into a hydrophobic pocket between helices A and B in a way that strikingly resembles part of the molecular interface in the U2AF heterodimer. This molecular recognition establishes a paradigm for protein binding by a subfamily of noncanonical RRMs.
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===Solution Structure of the third RNA Recognition Motif (RRM) of U2AF65 in complex with an N-terminal SF1 peptide===
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Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP.,Selenko P, Gregorovic G, Sprangers R, Stier G, Rhani Z, Kramer A, Sattler M Mol Cell. 2003 Apr;11(4):965-76. PMID:12718882<ref>PMID:12718882</ref>
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{{ABSTRACT_PUBMED_12718882}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1o0p" style="background-color:#fffaf0;"></div>
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[[1o0p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O0P OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:012718882</ref><references group="xtra"/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Gregorovic, G.]]
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[[Category: Large Structures]]
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[[Category: Kramer, A.]]
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[[Category: Gregorovic G]]
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[[Category: Rhani, Z.]]
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[[Category: Kramer A]]
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[[Category: Sattler, M.]]
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[[Category: Rhani Z]]
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[[Category: Selenko, P.]]
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[[Category: Sattler M]]
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[[Category: Sprangers, R.]]
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[[Category: Selenko P]]
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[[Category: Stier, G.]]
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[[Category: Sprangers R]]
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[[Category: 2 alpha helices additionally extended by a third helix c]]
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[[Category: Stier G]]
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[[Category: 4-stranded anti-parallel beta-sheet]]
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[[Category: Non-canonical rna recognition motif]]
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[[Category: Rna binding protein]]
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Current revision

Solution Structure of the third RNA Recognition Motif (RRM) of U2AF65 in complex with an N-terminal SF1 peptide

PDB ID 1o0p

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