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1o4z

From Proteopedia

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[[Image:1o4z.png|left|200px]]
 
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{{STRUCTURE_1o4z| PDB=1o4z | SCENE= }}
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==THE THREE-DIMENSIONAL STRUCTURE OF BETA-AGARASE B FROM ZOBELLIA GALACTANIVORANS==
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<StructureSection load='1o4z' size='340' side='right'caption='[[1o4z]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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===THE THREE-DIMENSIONAL STRUCTURE OF BETA-AGARASE B FROM ZOBELLIA GALACTANIVORANS===
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1o4z]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Zobellia_galactanivorans Zobellia galactanivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O4Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O4Z FirstGlance]. <br>
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{{ABSTRACT_PUBMED_12970344}}
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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==About this Structure==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o4z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o4z OCA], [https://pdbe.org/1o4z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o4z RCSB], [https://www.ebi.ac.uk/pdbsum/1o4z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o4z ProSAT]</span></td></tr>
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[[1o4z]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Zobellia_galactanivorans Zobellia galactanivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O4Z OCA].
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</table>
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== Function ==
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==Reference==
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[https://www.uniprot.org/uniprot/AGAB_ZOBGA AGAB_ZOBGA] Cleaves the beta-1,4-linkages between beta-D-galactose and alpha-L-3,6-anhydro-galactose residues in agarose. Cleaves agarose in a random manner with retention of the anomeric-bond configuration, producing beta-anomers that give rise progressively to alpha-anomers when mutarotation takes place. Also tolerant to hybrid substrates containing C6-sulfate groups at the -4, +1, and +3 positions.<ref>PMID:15456406</ref> <ref>PMID:22778272</ref>
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<ref group="xtra">PMID:012970344</ref><references group="xtra"/>
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== Evolutionary Conservation ==
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[[Category: Beta-agarase]]
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o4/1o4z_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o4z ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Zobellia galactanivorans]]
[[Category: Zobellia galactanivorans]]
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[[Category: Allouch, J.]]
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[[Category: Allouch J]]
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[[Category: Barbeyron, T.]]
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[[Category: Barbeyron T]]
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[[Category: Czjzek, M.]]
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[[Category: Czjzek M]]
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[[Category: Helbert, W.]]
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[[Category: Helbert W]]
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[[Category: Henrissat, B.]]
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[[Category: Henrissat B]]
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[[Category: Jam, M.]]
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[[Category: Jam M]]
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[[Category: Kloareg, B.]]
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[[Category: Kloareg B]]
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[[Category: 4-d-galactose linkage]]
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[[Category: Agarose degradation]]
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[[Category: Beta-agarase]]
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[[Category: Cleavage of beta-1]]
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[[Category: Glycoside hydrolase family 16]]
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[[Category: Hydrolase]]
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Current revision

THE THREE-DIMENSIONAL STRUCTURE OF BETA-AGARASE B FROM ZOBELLIA GALACTANIVORANS

PDB ID 1o4z

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