1kzf

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[[Image:1kzf.png|left|200px]]
 
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{{STRUCTURE_1kzf| PDB=1kzf | SCENE= }}
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==Crystal Structure of the Acyl-homoserine Lactone Synthase, EsaI==
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<StructureSection load='1kzf' size='340' side='right'caption='[[1kzf]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1kzf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pantoea_stewartii_subsp._stewartii Pantoea stewartii subsp. stewartii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KZF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KZF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kzf OCA], [https://pdbe.org/1kzf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kzf RCSB], [https://www.ebi.ac.uk/pdbsum/1kzf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kzf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ESAI_PANSE ESAI_PANSE] Required for the synthesis of OHHL (N-(3-oxohexanoyl)-L-homoserine lactone), an autoinducer molecule which binds to EsaR. OHHL is necessary for biosynthesis of EPS virulence factor (extracellular heteropolysaccharide) which plays a role in the development of Stewart's wilt on sweet corn.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kz/1kzf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kzf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Synthesis and detection of acyl-homoserine lactones (AHLs) enables many gram-negative bacteria to engage in quorum sensing, an intercellular signaling mechanism that activates differentiation to virulent and biofilm lifestyles. The AHL synthases catalyze acylation of S-adenosyl-L-methionine by acyl-acyl carrier protein and lactonization of the methionine moiety to give AHLs. The crystal structure of the AHL synthase, EsaI, determined at 1.8 A resolution, reveals a remarkable structural similarity to the N-acetyltransferases and defines a common phosphopantetheine binding fold as the catalytic core. Critical residues responsible for catalysis and acyl chain specificity have been identified from a modeled substrate complex and verified through functional analysis in vivo. A mechanism for the N-acylation of S-adenosyl-L-methionine by 3-oxo-hexanoyl-acyl carrier protein is proposed.
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===Crystal Structure of the Acyl-homoserine Lactone Synthase, EsaI===
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Structural basis and specificity of acyl-homoserine lactone signal production in bacterial quorum sensing.,Watson WT, Minogue TD, Val DL, von Bodman SB, Churchill ME Mol Cell. 2002 Mar;9(3):685-94. PMID:11931774<ref>PMID:11931774</ref>
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{{ABSTRACT_PUBMED_11931774}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1kzf" style="background-color:#fffaf0;"></div>
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[[1kzf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pantoea_stewartii_subsp._stewartii Pantoea stewartii subsp. stewartii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KZF OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:011931774</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pantoea stewartii subsp. stewartii]]
[[Category: Pantoea stewartii subsp. stewartii]]
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[[Category: Bodman, S Beck von.]]
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[[Category: Beck von Bodman S]]
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[[Category: Churchill, M E.A.]]
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[[Category: Churchill MEA]]
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[[Category: Minogue, T D.]]
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[[Category: Minogue TD]]
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[[Category: Val, D L.]]
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[[Category: Val DL]]
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[[Category: Watson, W T.]]
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[[Category: Watson WT]]
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[[Category: Acylhomoserine lactone]]
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[[Category: Alpha-beta]]
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[[Category: Autoinducer synthase]]
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[[Category: Bacterial pathogenesis]]
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[[Category: Ligase]]
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[[Category: Quorum sensing]]
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Current revision

Crystal Structure of the Acyl-homoserine Lactone Synthase, EsaI

PDB ID 1kzf

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