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1kou

From Proteopedia

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[[Image:1kou.png|left|200px]]
 
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{{STRUCTURE_1kou| PDB=1kou | SCENE= }}
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==Crystal Structure of the Photoactive Yellow Protein Reconstituted with Caffeic Acid at 1.16 A Resolution==
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<StructureSection load='1kou' size='340' side='right'caption='[[1kou]], [[Resolution|resolution]] 1.16&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1kou]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KOU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.16&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DHC:CAFFEIC+ACID'>DHC</scene>, <scene name='pdbligand=NBU:N-BUTANE'>NBU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kou OCA], [https://pdbe.org/1kou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kou RCSB], [https://www.ebi.ac.uk/pdbsum/1kou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kou ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PYP_HALHA PYP_HALHA] Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ko/1kou_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kou ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A structural study is described of the photoactive yellow protein (PYP) reconstituted with the chromophore derivative 3,4-dihydroxycinnamic acid. The crystal structure of PYP reconstituted with this chromophore at 1.16 A resolution is reported in space group P6(5). This is the first high-resolution structure of a photoreceptor containing a modified chromophore. The introduction of an extra hydroxyl group in the native chromophore (i.e. p-coumaric acid) appears to perturb the structure of the hybrid yellow protein only slightly. The chromophore is bound by the protein in two different conformations, separated by a rotation of 180 degrees of the catechol ring. In combination with available spectroscopic data, it is concluded that the caffeic acid chromophore binds to the protein in a strained conformation, which leads to a faster ejection from the chromophore-binding pocket upon pB formation.
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===Crystal Structure of the Photoactive Yellow Protein Reconstituted with Caffeic Acid at 1.16 A Resolution===
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Structure of the photoactive yellow protein reconstituted with caffeic acid at 1.16 A resolution.,van Aalten DM, Crielaard W, Hellingwerf KJ, Joshua-Tor L Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):585-90. Epub 2002, Mar 22. PMID:11914481<ref>PMID:11914481</ref>
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{{ABSTRACT_PUBMED_11914481}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1kou" style="background-color:#fffaf0;"></div>
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[[1kou]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KOU OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:011914481</ref><references group="xtra"/>
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</StructureSection>
[[Category: Halorhodospira halophila]]
[[Category: Halorhodospira halophila]]
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[[Category: Aalten, D M.F van.]]
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[[Category: Large Structures]]
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[[Category: Crielaard, W.]]
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[[Category: Crielaard W]]
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[[Category: Hellingwerf, K J.]]
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[[Category: Hellingwerf KJ]]
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[[Category: Joshua-Tor, L.]]
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[[Category: Joshua-Tor L]]
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[[Category: Photoreceptor]]
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[[Category: Van Aalten DMF]]
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[[Category: Photosynthesis]]
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Current revision

Crystal Structure of the Photoactive Yellow Protein Reconstituted with Caffeic Acid at 1.16 A Resolution

PDB ID 1kou

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