1rtt

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:25, 14 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1rtt.gif|left|200px]]<br /><applet load="1rtt" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1rtt, resolution 1.28&Aring;" />
 
-
'''Crystal structure determination of a putative NADH-dependent reductase using sulfur anomalous signal'''<br />
 
-
==Overview==
+
==Crystal structure determination of a putative NADH-dependent reductase using sulfur anomalous signal==
-
The availability of high-intensity synchrotron facilities, technological advances in data-collection techniques and improved data-reduction and crystallographic software have ushered in a new era in high-throughput macromolecular crystallography. Here, the de novo automated crystal structure determination at 1.28 A resolution of an NAD(P)H-dependent FMN reductase flavoprotein from Pseudomonas aeruginosa PA01-derived protein Q9I4D4 using the anomalous signal from an unusually small number of S atoms is reported. Although this protein lacks the flavodoxin key fingerprint motif [(T/S)XTGXT], it has been confirmed to bind flavin mononucleotide and the binding site was identified via X-ray crystallography. This protein contains a novel flavin mononucleotide-binding site GSLRSGSYN, which has not been previously reported. Detailed statistics pertaining to sulfur phasing and other factors contributing to structure determination are discussed. Structural comparisons of the apoenzyme and the protein complexed with flavin mononucleotide show conformational changes on cofactor binding. NADPH-dependent activity has been confirmed with biochemical assays.
+
<StructureSection load='1rtt' size='340' side='right'caption='[[1rtt]], [[Resolution|resolution]] 1.28&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1rtt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RTT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RTT FirstGlance]. <br>
-
1RTT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RTT OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.28&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rtt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rtt OCA], [https://pdbe.org/1rtt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rtt RCSB], [https://www.ebi.ac.uk/pdbsum/1rtt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rtt ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1rtt TOPSAN]</span></td></tr>
-
Structure determination of an FMN reductase from Pseudomonas aeruginosa PA01 using sulfur anomalous signal., Agarwal R, Bonanno JB, Burley SK, Swaminathan S, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):383-91. Epub 2006, Mar 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16552139 16552139]
+
</table>
-
[[Category: Pseudomonas aeruginosa pao1]]
+
== Function ==
-
[[Category: Single protein]]
+
[https://www.uniprot.org/uniprot/FMNRE_PSEAE FMNRE_PSEAE] Has NAD(P)H-dependent FMN reductase activity.<ref>PMID:16552139</ref>
-
[[Category: Agarwal, R.]]
+
== Evolutionary Conservation ==
-
[[Category: Burley, S K.]]
+
[[Image:Consurf_key_small.gif|200px|right]]
-
[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
+
Check<jmol>
-
[[Category: Swaminathan, S.]]
+
<jmolCheckbox>
-
[[Category: SO4]]
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rt/1rtt_consurf.spt"</scriptWhenChecked>
-
[[Category: new york structural genomix research consortium]]
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
[[Category: nysgxrc]]
+
<text>to colour the structure by Evolutionary Conservation</text>
-
[[Category: protein structure initiative]]
+
</jmolCheckbox>
-
[[Category: psi]]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rtt ConSurf].
-
[[Category: putative reductase]]
+
<div style="clear:both"></div>
-
[[Category: sad with sulfur]]
+
== References ==
-
[[Category: structural genomics]]
+
<references/>
-
 
+
__TOC__
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:54:29 2008''
+
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Pseudomonas aeruginosa PAO1]]
 +
[[Category: Agarwal R]]
 +
[[Category: Burley SK]]
 +
[[Category: Swaminathan S]]

Current revision

Crystal structure determination of a putative NADH-dependent reductase using sulfur anomalous signal

PDB ID 1rtt

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rtt"
Personal tools