1m32

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[[Image:1m32.png|left|200px]]
 
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{{STRUCTURE_1m32| PDB=1m32 | SCENE= }}
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==Crystal Structure of 2-aminoethylphosphonate Transaminase==
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<StructureSection load='1m32' size='340' side='right'caption='[[1m32]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1m32]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M32 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M32 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=POA:PHOSPHONOACETALDEHYDE'>POA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m32 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m32 OCA], [https://pdbe.org/1m32 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m32 RCSB], [https://www.ebi.ac.uk/pdbsum/1m32 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m32 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHNW_SALTY PHNW_SALTY] Involved in phosphonate degradation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m3/1m32_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m32 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phosphonates allow certain organisms to thrive in otherwise hostile environments, and 2-aminoethylphosphonate (AEP) is a precursor of many cellular phosphonates. AEP transaminase (AEPT) is an enzyme essential to phosphonate synthesis and degradation pathways. The crystal structure of AEP transaminase was determined by multiwavelength anomalous diffraction of 66 selenium atoms. The refined structure at 2.2 A resolution revealed an overall fold and active site location similar to those of the dimeric, two-domain structure of type I aminotransferases. The active site contains a cofactor, pyridoxal 5'-phosphate (PLP), and the product phosphonoacetaldehyde. Comparison with other type I aminotransferase structures shows that the PLP-protein interactions are conserved. Modeling of bound substrates and products reveals the structural basis for AEP recognition and the stereospecificity of proton elimination at the alpha-carbon and indicates conformational changes along the reaction pathway.
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===Crystal Structure of 2-aminoethylphosphonate Transaminase===
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Degradation pathway of the phosphonate ciliatine: crystal structure of 2-aminoethylphosphonate transaminase.,Chen CC, Zhang H, Kim AD, Howard A, Sheldrick GM, Mariano-Dunaway D, Herzberg O Biochemistry. 2002 Nov 5;41(44):13162-9. PMID:12403617<ref>PMID:12403617</ref>
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{{ABSTRACT_PUBMED_12403617}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1m32" style="background-color:#fffaf0;"></div>
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[[1m32]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M32 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:012403617</ref><references group="xtra"/>
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</StructureSection>
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[[Category: 2-aminoethylphosphonate--pyruvate transaminase]]
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[[Category: Large Structures]]
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[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Chen, C C.H.]]
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[[Category: Chen CCH]]
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[[Category: Herzberg, O.]]
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[[Category: Herzberg O]]
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[[Category: Howard, A.]]
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[[Category: Howard A]]
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[[Category: Kim, A D.]]
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[[Category: Kim AD]]
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[[Category: Mariano-Dunnaway, D.]]
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[[Category: Mariano-Dunnaway D]]
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[[Category: Sheldrick, G M.]]
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[[Category: Sheldrick GM]]
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[[Category: Zhang, H.]]
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[[Category: Zhang H]]
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[[Category: Plp-dependent aminotransferase fold]]
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[[Category: Transferase]]
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Current revision

Crystal Structure of 2-aminoethylphosphonate Transaminase

PDB ID 1m32

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