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1rwa

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[[Image:1rwa.gif|left|200px]]<br /><applet load="1rwa" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rwa, resolution 1.30&Aring;" />
 
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'''Crystal structure of Arthrobacter aurescens chondroitin AC lyase'''<br />
 
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==Overview==
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==Crystal structure of Arthrobacter aurescens chondroitin AC lyase==
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Chondroitin lyases (EC 4.2.2.4 and EC 4.2.2.5) are glycosaminoglycan-degrading enzymes that act as eliminases. Chondroitin lyase AC from Arthrobacter aurescens (ArthroAC) is known to act on chondroitin 4-sulfate and chondroitin 6-sulfate but not on dermatan sulfate. Like other chondroitin AC lyases, it is capable of cleaving hyaluronan. We have determined the three-dimensional crystal structure of ArthroAC in its native form as well as in complex with its substrates (chondroitin 4-sulfate tetrasaccharide, CS(tetra) and hyaluronan tetrasaccharide) at resolution varying from 1.25 A to 1.9A. The primary sequence of ArthroAC has not been previously determined but it was possible to determine the amino acid sequence of this enzyme from the high-resolution electron density maps and to confirm it by mass spectrometry. The enzyme-substrate complexes were obtained by soaking the substrate into the crystals for varying lengths of time (30 seconds to ten hours) and flash-cooling the crystals. The electron density map for crystals soaked in the substrate for as short as 30 seconds showed the substrate clearly and indicated that the ring of central glucuronic acid assumes a distorted boat conformation. This structure strongly supports the lytic mechanism where Tyr242 acts as a general base that abstracts the proton from the C5 position of glucuronic acid while Asn183 and His233 neutralize the charge on the glucuronate acidic group. Comparison of this structure with that of chondroitinase AC from Flavobacterium heparinum (FlavoAC) provides an explanation for the exolytic and endolytic mode of action of ArthroAC and FlavoAC, respectively.
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<StructureSection load='1rwa' size='340' side='right'caption='[[1rwa]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1rwa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Paenarthrobacter_aurescens Paenarthrobacter aurescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RWA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RWA FirstGlance]. <br>
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1RWA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arthrobacter_aurescens Arthrobacter aurescens] with <scene name='pdbligand=HG:'>HG</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RWA OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rwa OCA], [https://pdbe.org/1rwa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rwa RCSB], [https://www.ebi.ac.uk/pdbsum/1rwa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rwa ProSAT]</span></td></tr>
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High-resolution crystal structure of Arthrobacter aurescens chondroitin AC lyase: an enzyme-substrate complex defines the catalytic mechanism., Lunin VV, Li Y, Linhardt RJ, Miyazono H, Kyogashima M, Kaneko T, Bell AW, Cygler M, J Mol Biol. 2004 Mar 19;337(2):367-86. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15003453 15003453]
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</table>
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[[Category: Arthrobacter aurescens]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/P84141_PAEAU P84141_PAEAU]
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[[Category: Bell, A W.]]
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== Evolutionary Conservation ==
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[[Category: Cygler, M.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Kyogashima, M.]]
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Check<jmol>
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[[Category: Li, Y.]]
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<jmolCheckbox>
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[[Category: Lunin, V V.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rw/1rwa_consurf.spt"</scriptWhenChecked>
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[[Category: Miyazono, H.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: GOL]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: HG]]
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</jmolCheckbox>
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[[Category: chondroitin lyase]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rwa ConSurf].
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[[Category: chondroitinase]]
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<div style="clear:both"></div>
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__TOC__
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:55:11 2008''
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Paenarthrobacter aurescens]]
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[[Category: Bell AW]]
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[[Category: Cygler M]]
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[[Category: Kyogashima M]]
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[[Category: Li Y]]
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[[Category: Lunin VV]]
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[[Category: Miyazono H]]

Current revision

Crystal structure of Arthrobacter aurescens chondroitin AC lyase

PDB ID 1rwa

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