1rwi

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[[Image:1rwi.gif|left|200px]]<br /><applet load="1rwi" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rwi, resolution 1.8&Aring;" />
 
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'''Extracellular domain of Mycobacterium tuberculosis PknD'''<br />
 
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==Overview==
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==Extracellular domain of Mycobacterium tuberculosis PknD==
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Diverse pathogenic bacteria produce transmembrane receptor Ser/Thr protein kinases (STPKs), but little is known about the signals mediated by these "eukaryotic-like" proteins. To explore the basis for signaling in the bacterial STPK receptor family, we determined the structure of the sensor domain of Mycobacterium tuberculosis PknD. In two crystal forms, the PknD sensor domain forms a rigid, six-bladed beta-propeller with a flexible tether to the transmembrane domain. The PknD sensor domain is the most symmetric beta-propeller structure described. All residues that vary most among the blade subdomains cluster in the large "cup" motif, analogous to the ligand-binding surface in many beta-propeller proteins. These results suggest that PknD binds a multivalent ligand that signals by changing the quaternary structure of the intracellular kinase domain.
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<StructureSection load='1rwi' size='340' side='right'caption='[[1rwi]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rwi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RWI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RWI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rwi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rwi OCA], [https://pdbe.org/1rwi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rwi RCSB], [https://www.ebi.ac.uk/pdbsum/1rwi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rwi ProSAT], [https://www.topsan.org/Proteins/TBSGC/1rwi TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PKND_MYCTU PKND_MYCTU] Key microbial factor required for central nervous system tuberculosis. Required for invasion of host brain endothelia, but not macrophages, lung epithelia or other endothelia. Phosphorylates the anti-anti-sigma factor homolog Rv0516c, which inhibits binding of Rv0516c to Rv2638, another anti-anti-sigma factor. Can also phosphorylate the FHA domain of Rv1747.<ref>PMID:15987910</ref> <ref>PMID:17242402</ref> <ref>PMID:17411339</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rw/1rwi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rwi ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RWI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RWI OCA].
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*[[Serine/threonine protein kinase 3D structures|Serine/threonine protein kinase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Sensor domain of the Mycobacterium tuberculosis receptor Ser/Thr protein kinase, PknD, forms a highly symmetric beta propeller., Good MC, Greenstein AE, Young TA, Ng HL, Alber T, J Mol Biol. 2004 May 28;339(2):459-69. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15136047 15136047]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Non-specific serine/threonine protein kinase]]
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[[Category: Alber T]]
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[[Category: Single protein]]
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[[Category: Good MC]]
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[[Category: Alber, T.]]
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[[Category: Greenstein AE]]
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[[Category: Good, M C.]]
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[[Category: Ng HL]]
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[[Category: Greenstein, A E.]]
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[[Category: Young TA]]
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[[Category: Ng, H L.]]
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[[Category: TBSGC, TB Structural Genomics Consortium.]]
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[[Category: Young, T A.]]
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[[Category: CD]]
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[[Category: beta propeller]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomics]]
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[[Category: tb structural genomics consortium]]
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[[Category: tbsgc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:55:16 2008''
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Current revision

Extracellular domain of Mycobacterium tuberculosis PknD

PDB ID 1rwi

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OCA

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