1mm8

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[[Image:1mm8.png|left|200px]]
 
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{{STRUCTURE_1mm8| PDB=1mm8 | SCENE= }}
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==Crystal structure of Tn5 Transposase complexed with ME DNA==
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<StructureSection load='1mm8' size='340' side='right'caption='[[1mm8]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1mm8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MM8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mm8 OCA], [https://pdbe.org/1mm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mm8 RCSB], [https://www.ebi.ac.uk/pdbsum/1mm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mm8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TN5P_ECOLX TN5P_ECOLX] Mediates transposition of transposon Tn5 by a 'cut and paste' mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA-bound transposase creates a synaptic DNA complex. After nicking of the first DNA strand, excision of the transposon proceeds through a series of intermediates. The transposase then mediates the insertion of the transposon at a new site by strand transfer. The activity of the wild-type transposase is very low, and is further inhibited by dimerization with the transposase inhibitor (inh).<ref>PMID:6260374</ref> <ref>PMID:6291786</ref> <ref>PMID:6303899</ref> <ref>PMID:1310499</ref> <ref>PMID:8226636</ref> <ref>PMID:8871560</ref> <ref>PMID:11877443</ref> <ref>PMID:12367522</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mm/1mm8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mm8 ConSurf].
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<div style="clear:both"></div>
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===Crystal structure of Tn5 Transposase complexed with ME DNA===
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==See Also==
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*[[Transposase 3D structures|Transposase 3D structures]]
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{{ABSTRACT_PUBMED_12367522}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[1mm8]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MM8 OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:012367522</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Bhasin, A.]]
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[[Category: Large Structures]]
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[[Category: Lovell, S.]]
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[[Category: Bhasin A]]
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[[Category: Rayment, I.]]
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[[Category: Lovell S]]
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[[Category: Reznikoff, W S.]]
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[[Category: Rayment I]]
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[[Category: Steiniger-White, M.]]
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[[Category: Reznikoff WS]]
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[[Category: Protein-dna complex]]
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[[Category: Steiniger-White M]]
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[[Category: Transcription-dna complex]]
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Current revision

Crystal structure of Tn5 Transposase complexed with ME DNA

PDB ID 1mm8

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OCA

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