1rzy

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[[Image:1rzy.jpg|left|200px]]<br /><applet load="1rzy" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rzy, resolution 1.80&Aring;" />
 
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'''Crystal structure of rabbit Hint complexed with N-ethylsulfamoyladenosine'''<br />
 
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==Overview==
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==Crystal structure of rabbit Hint complexed with N-ethylsulfamoyladenosine==
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Hint, histidine triad nucleotide-binding protein, is a universally conserved enzyme that hydrolyzes AMP linked to lysine and, in yeast, functions as a positive regulator of the RNA polymerase II C-terminal domain kinase, Kin28. To explore the biochemical and structural bases for the adenosine phosphoramidate hydrolase activity of rabbit Hint, we synthesized novel substrates linking a p-nitroaniline group to adenylate (AMP-pNA) and inhibitors that consist of an adenosine group and 5'-sulfamoyl (AdoOSO(2)NH(2)) or N-ethylsulfamoyl (AdoOSO(2)NHCH(2)CH(3)) group. AMP-pNA is a suitable substrate for Hint that allowed characterization of the inhibitors; titration of each inhibitor into AMP-pNA assays revealed their K(i) values. The N-ethylsulfamoyl derivative has a 13-fold binding advantage over the sulfamoyl adenosine. The 1.8-A cocrystal structure of rabbit Hint with N-ethylsulfamoyl adenosine revealed a binding site for the ethyl group against Trp-123, a residue that reaches across the Hint dimer interface to interact with the alkyl portion of the inhibitor and, presumably, the alkyl portion of a lysyl substrate. Ser-107 is positioned to donate a hydrogen bond to the leaving group nitrogen. Consistent with a role in acid-base catalysis, the Hint S107A mutant protein displayed depressed catalytic activity.
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<StructureSection load='1rzy' size='340' side='right'caption='[[1rzy]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rzy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RZY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5AS:5-O-(N-ETHYL-SULFAMOYL)ADENOSINE'>5AS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rzy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rzy OCA], [https://pdbe.org/1rzy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rzy RCSB], [https://www.ebi.ac.uk/pdbsum/1rzy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rzy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HINT1_RABIT HINT1_RABIT] Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rz/1rzy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rzy ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RZY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with <scene name='pdbligand=5AS:'>5AS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZY OCA].
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*[[Histidine triad nucleotide-binding protein 3D structures|Histidine triad nucleotide-binding protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Biochemical, crystallographic, and mutagenic characterization of hint, the AMP-lysine hydrolase, with novel substrates and inhibitors., Krakowiak A, Pace HC, Blackburn GM, Adams M, Mekhalfia A, Kaczmarek R, Baraniak J, Stec WJ, Brenner C, J Biol Chem. 2004 Apr 30;279(18):18711-6. Epub 2004 Feb 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14982931 14982931]
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[[Category: Large Structures]]
[[Category: Oryctolagus cuniculus]]
[[Category: Oryctolagus cuniculus]]
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[[Category: Single protein]]
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[[Category: Adams M]]
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[[Category: Adams, M.]]
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[[Category: Baraniak J]]
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[[Category: Baraniak, J.]]
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[[Category: Blackburn GM]]
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[[Category: Blackburn, G M.]]
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[[Category: Brenner C]]
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[[Category: Brenner, C.]]
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[[Category: Kaczmarek R]]
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[[Category: Kaczmarek, R.]]
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[[Category: Krakowiak AK]]
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[[Category: Krakowiak, A K.]]
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[[Category: Mekhalfia A]]
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[[Category: Mekhalfia, A.]]
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[[Category: Pace HC]]
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[[Category: Pace, H C.]]
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[[Category: Stec WJ]]
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[[Category: Stec, W J.]]
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[[Category: 5AS]]
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[[Category: hit protein; protein-inhibitor complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:56:26 2008''
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Current revision

Crystal structure of rabbit Hint complexed with N-ethylsulfamoyladenosine

PDB ID 1rzy

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