1s1l

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:27, 14 February 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1s1l.gif|left|200px]]<br /><applet load="1s1l" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1s1l, resolution 2.20&Aring;" />
 
-
'''Influence of Groove Interactions on the Formation of DNA Holliday Junctions'''<br />
 
-
==Overview==
+
==Influence of Groove Interactions on the Formation of DNA Holliday Junctions==
-
The inosine-containing sequence d(CCIGTACm(5)CGG) is shown to crystallize as a four-stranded DNA junction. This structure is nearly identical to the antiparallel junction formed by the parent d(CCGGTACm(5)()CGG) sequence [Vargason, J. M., and Ho, P. S. (2002) J. Biol. Chem. 277, 21041-21049] in terms of its conformational geometry, and inter- and intramolecular interactions within the DNA and between the DNA and solvent, even though the 2-amino group in the minor groove of the important G(3).m(5)C(8) base pair of the junction core trinucleotide (italicized) has been removed. In contrast, the analogous 2,6-diaminopurine sequence d(CCDGTACTGG) crystallizes as resolved duplex DNAs, just like its parent sequence d(CCAGTACTGG) [Hays, F. A., Vargason, J. M., and Ho, P. S. (2003) Biochemistry 42, 9586-9597]. These results demonstrate that it is not the presence or absence of the 2-amino group in the minor groove of the R(3).Y(8) base pair that specifies whether a sequence forms a junction, but the positions of the extracyclic amino and keto groups in the major groove. Finally, the study shows that the arms of the junction can accommodate perturbations to the B-DNA conformation of the stacked duplex arms associated with the loss of the 2-amino substituent, and that two hydrogen bonding interactions from the C(7) and Y(8) pyrimidine nucleotides to phosphate oxygens of the junction crossover specify the geometry of the Holliday junction.
+
<StructureSection load='1s1l' size='340' side='right'caption='[[1s1l]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1s1l]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S1L FirstGlance]. <br>
-
1S1L is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S1L OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s1l OCA], [https://pdbe.org/1s1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s1l RCSB], [https://www.ebi.ac.uk/pdbsum/1s1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s1l ProSAT]</span></td></tr>
-
Influence of minor groove substituents on the structure of DNA Holliday junctions., Hays FA, Jones ZJ, Ho PS, Biochemistry. 2004 Aug 3;43(30):9813-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15274635 15274635]
+
</table>
-
[[Category: Protein complex]]
+
__TOC__
-
[[Category: Hays, F A.]]
+
</StructureSection>
-
[[Category: Ho, P S.]]
+
[[Category: Large Structures]]
-
[[Category: Jones, Z J.]]
+
[[Category: Hays FA]]
-
[[Category: dna four-way junction]]
+
[[Category: Ho PS]]
-
[[Category: holliday junction]]
+
[[Category: Jones ZJ]]
-
[[Category: inosine]]
+
-
[[Category: major groove]]
+
-
[[Category: minor groove]]
+
-
[[Category: x-ray diffraction]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:56:59 2008''
+

Current revision

Influence of Groove Interactions on the Formation of DNA Holliday Junctions

PDB ID 1s1l

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1s1l"
Personal tools