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1s2o

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[[Image:1s2o.gif|left|200px]]<br /><applet load="1s2o" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1s2o, resolution 1.4&Aring;" />
 
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'''X-Ray structure of the sucrose-phosphatase (SPP) from Synechocystis sp. PCC6803 at 1.40 A resolution'''<br />
 
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==Overview==
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==X-Ray structure of the sucrose-phosphatase (SPP) from Synechocystis sp. PCC6803 at 1.40 A resolution==
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Sucrose-phosphatase (SPP) catalyzes the final step in the pathway of sucrose biosynthesis in both plants and cyanobacteria, and the SPPs from these two groups of organisms are closely related. We have crystallized the enzyme from the cyanobacterium Synechocystis sp PCC 6803 and determined its crystal structure alone and in complex with various ligands. The protein consists of a core domain containing the catalytic site and a smaller cap domain that contains a glucose binding site. Two flexible hinge loops link the two domains, forming a structure that resembles a pair of sugar tongs. The glucose binding site plays a major role in determining the enzyme's remarkable substrate specificity and is also important for its inhibition by sucrose and glucose. It is proposed that the catalytic reaction is initiated by nucleophilic attack on the substrate by Asp9 and involves formation of a covalent phospho-Asp9-enzyme intermediate. From modeling based on the SPP structure, we predict that the noncatalytic SPP-like domain of the Synechocystis sucrose-phosphate synthase could bind sucrose-6(F)-phosphate and propose that this domain might be involved in metabolite channeling between the last two enzymes in the pathway of sucrose synthesis.
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<StructureSection load='1s2o' size='340' side='right'caption='[[1s2o]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1s2o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S2O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S2O FirstGlance]. <br>
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1S2O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Sucrose-phosphatase Sucrose-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.24 3.1.3.24] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S2O OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s2o OCA], [https://pdbe.org/1s2o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s2o RCSB], [https://www.ebi.ac.uk/pdbsum/1s2o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s2o ProSAT]</span></td></tr>
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The structure of a cyanobacterial sucrose-phosphatase reveals the sugar tongs that release free sucrose in the cell., Fieulaine S, Lunn JE, Borel F, Ferrer JL, Plant Cell. 2005 Jul;17(7):2049-58. Epub 2005 Jun 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15937230 15937230]
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</table>
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[[Category: Single protein]]
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== Function ==
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[[Category: Sucrose-phosphatase]]
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[https://www.uniprot.org/uniprot/P74325_SYNY3 P74325_SYNY3]
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[[Category: Synechocystis sp.]]
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== Evolutionary Conservation ==
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[[Category: Borel, F.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Ferrer, J L.]]
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Check<jmol>
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[[Category: Fieulaine, S.]]
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<jmolCheckbox>
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[[Category: Lunn, J E.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s2/1s2o_consurf.spt"</scriptWhenChecked>
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[[Category: MG]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: cyanobacteria]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: had superfamily]]
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</jmolCheckbox>
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[[Category: phosphohydrolase]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s2o ConSurf].
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[[Category: sucrose]]
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<div style="clear:both"></div>
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__TOC__
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:57:18 2008''
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Synechocystis sp. PCC 6803]]
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[[Category: Borel F]]
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[[Category: Ferrer JL]]
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[[Category: Fieulaine S]]
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[[Category: Lunn JE]]

Current revision

X-Ray structure of the sucrose-phosphatase (SPP) from Synechocystis sp. PCC6803 at 1.40 A resolution

PDB ID 1s2o

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