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1s45

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[[Image:1s45.gif|left|200px]]<br /><applet load="1s45" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1s45, resolution 2.20&Aring;" />
 
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'''Crystal structure analysis of the DNA quadruplex d(TGGGGT) S1'''<br />
 
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==Overview==
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==Crystal structure analysis of the DNA quadruplex d(TGGGGT) S1==
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We report two new structures of the quadruplex d(TGGGGT)4 obtained by single crystal X-ray diffraction. In one of them a thymine tetrad is found. Thus the yeast telomere sequences d(TG1-3) might be able to form continuous quadruplex structures, involving both guanine and thymine tetrads. Our study also shows substantial differences in the arrangement of thymines when compared with previous studies. We find five different types of organization: (i) groove binding with hydrogen bonds to guanines from a neighbour quadruplex; (ii) partially ordered groove binding, without any hydrogen bond; (iii) stacked thymine triads, formed at the 3'ends of the quadruplexes; (iv) a thymine tetrad between two guanine tetrads. Thymines are stabilized in pairs by single hydrogen bonds. A central sodium ion interacts with two thymines and contributes to the tetrad structure. (v) Completely disordered thymines which do not show any clear location in the crystal. The tetrads are stabilized by either Na+ or Tl+ ions. We show that by using MAD methods, Tl+ can be unambiguously located and distinguished from Na+. We can thus determine the preference for either ion in each ionic site of the structure under the conditions used by us.
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<StructureSection load='1s45' size='340' side='right'caption='[[1s45]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1s45]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S45 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S45 FirstGlance]. <br>
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1S45 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=TL:'>TL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S45 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TL:THALLIUM+(I)+ION'>TL</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s45 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s45 OCA], [https://pdbe.org/1s45 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s45 RCSB], [https://www.ebi.ac.uk/pdbsum/1s45 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s45 ProSAT]</span></td></tr>
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A thymine tetrad in d(TGGGGT) quadruplexes stabilized with Tl+/Na+ ions., Caceres C, Wright G, Gouyette C, Parkinson G, Subirana JA, Nucleic Acids Res. 2004 Feb 11;32(3):1097-102. Print 2004. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14960719 14960719]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Caceres, C.]]
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</StructureSection>
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[[Category: Gouyette, C.]]
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[[Category: Large Structures]]
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[[Category: Parkinson, G.]]
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[[Category: Caceres C]]
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[[Category: Subirana, J A.]]
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[[Category: Gouyette C]]
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[[Category: Wright, G.]]
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[[Category: Parkinson G]]
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[[Category: MG]]
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[[Category: Subirana JA]]
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[[Category: NA]]
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[[Category: Wright G]]
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[[Category: TL]]
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[[Category: quadruplex dna]]
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[[Category: thallium]]
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[[Category: thymine triads]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:57:46 2008''
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Current revision

Crystal structure analysis of the DNA quadruplex d(TGGGGT) S1

PDB ID 1s45

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