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1sfu

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[[Image:1sfu.gif|left|200px]]<br /><applet load="1sfu" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sfu, resolution 2.0&Aring;" />
 
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'''Crystal structure of the viral Zalpha domain bound to left-handed Z-DNA'''<br />
 
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==Overview==
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==Crystal structure of the viral Zalpha domain bound to left-handed Z-DNA==
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A conserved feature of poxviruses is a protein, well characterized as E3L in vaccinia virus, that confers IFN resistance on the virus. This protein comprises two domains, an N-terminal Z-DNA-binding protein domain (Zalpha) and a C-terminal double-stranded RNA-binding domain. Both are required for pathogenicity of vaccinia virus in mice infected by intracranial injection. Here, we describe the crystal structure of the Zalpha domain from the E3L-like protein of Yaba-like disease virus, a Yatapoxvirus, in a complex with Z-DNA, solved at a 2.0-A resolution. The DNA contacting surface of Yaba-like disease virus Zalpha(E3L) closely resembles that of other structurally defined members of the Zalpha family, although some variability exists in the beta-hairpin region. In contrast to the Z-DNA-contacting surface, the nonbinding surface of members of the Zalpha family are unrelated; this surface may effect protein-specific interactions. The presence of the conserved and tailored Z-DNA-binding surface, which interacts specifically with the zigzag backbone and syn base diagnostic of the Z-form, reinforces the importance to poxvirus infection of the ability of this protein to recognize the Z-conformation.
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<StructureSection load='1sfu' size='340' side='right'caption='[[1sfu]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sfu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Yaba-like_disease_virus Yaba-like disease virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SFU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sfu OCA], [https://pdbe.org/1sfu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sfu RCSB], [https://www.ebi.ac.uk/pdbsum/1sfu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sfu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/E3_YLDV E3_YLDV] RNA-binding protein that plays a role in the inhibition of multiple cellular antiviral responses activated by double-stranded RNA (dsRNA), such as inhibition of PKR activation, necroptosis, and IFN-mediated antiviral activities (By similarity). Recognizes and binds Z-RNA structures via its Z-binding domain and dsRNA via its DRBM domain: RNA-binding activity is required to escape host ZBP1-dependent necroptosis (By similarity). Mechanistically, the Z-binding domain binds Z-RNAs that are produced during Yaba-like disease virus infection, thereby competing with Z-RNA detection by host ZBP1, suppressing ZBP1-dependent necroptosis (By similarity).[UniProtKB:P21605]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sf/1sfu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sfu ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SFU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SFU OCA].
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*[[Z-DNA|Z-DNA]]
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__TOC__
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==Reference==
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</StructureSection>
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A poxvirus protein forms a complex with left-handed Z-DNA: crystal structure of a Yatapoxvirus Zalpha bound to DNA., Ha SC, Lokanath NK, Van Quyen D, Wu CA, Lowenhaupt K, Rich A, Kim YG, Kim KK, Proc Natl Acad Sci U S A. 2004 Oct 5;101(40):14367-72. Epub 2004 Sep 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15448208 15448208]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Yaba-like disease virus]]
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[[Category: Viruses]]
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[[Category: Ha SC]]
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[[Category: Ha, S C.]]
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[[Category: Kim KK]]
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[[Category: Kim, K K.]]
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[[Category: Kim YG]]
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[[Category: Kim, Y G.]]
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[[Category: Lowenhaupt K]]
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[[Category: Lowenhaupt, K.]]
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[[Category: Rich A]]
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[[Category: Quyen, D Van.]]
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[[Category: Van Quyen D]]
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[[Category: Rich, A.]]
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[[Category: Wu CA]]
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[[Category: Wu, C A.]]
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[[Category: protein/z-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:01:07 2008''
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Current revision

Crystal structure of the viral Zalpha domain bound to left-handed Z-DNA

PDB ID 1sfu

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