1si7

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[[Image:1si7.jpg|left|200px]]<br /><applet load="1si7" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1si7, resolution 2.20&Aring;" />
 
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'''Structure of E. coli tRNA psi 13 pseudouridine synthase TruD'''<br />
 
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==Overview==
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==Structure of E. coli tRNA psi 13 pseudouridine synthase TruD==
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TruD, a recently discovered novel pseudouridine synthase in Escherichia coli, is responsible for modifying uridine13 in tRNA(Glu) to pseudouridine. It has little sequence homology with the other 10 pseudouridine synthases in E. coli which themselves have been grouped into four related protein families. Crystal structure determination of TruD revealed a two domain structure consisting of a catalytic domain that differs in sequence but is structurally very similar to the catalytic domain of other pseudouridine synthases and a second large domain (149 amino acids, 43% of total) with a novel alpha/beta fold that up to now has not been found in any other protein.
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<StructureSection load='1si7' size='340' side='right'caption='[[1si7]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1si7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SI7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1si7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1si7 OCA], [https://pdbe.org/1si7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1si7 RCSB], [https://www.ebi.ac.uk/pdbsum/1si7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1si7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRUD_ECOLI TRUD_ECOLI] Responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs.<ref>PMID:12756329</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/si/1si7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1si7 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SI7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SI7 OCA].
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*[[Guide-independent Pseudouridine synthase|Guide-independent Pseudouridine synthase]]
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*[[Pseudouridine synthase 3D structures|Pseudouridine synthase 3D structures]]
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==Reference==
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== References ==
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Crystal structure of TruD, a novel pseudouridine synthase with a new protein fold., Kaya Y, Del Campo M, Ofengand J, Malhotra A, J Biol Chem. 2004 Apr 30;279(18):18107-10. Epub 2004 Mar 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14999002 14999002]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Pseudouridylate synthase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Del Campo M]]
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[[Category: Campo, M Del.]]
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[[Category: Kaya Y]]
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[[Category: Kaya, Y.]]
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[[Category: Malhotra A]]
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[[Category: Malhotra, A.]]
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[[Category: Ofengand J]]
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[[Category: Ofengand, J.]]
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[[Category: novel fold]]
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[[Category: pseudouridine synthase]]
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[[Category: trna]]
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[[Category: trud]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:01:45 2008''
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Current revision

Structure of E. coli tRNA psi 13 pseudouridine synthase TruD

PDB ID 1si7

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