1sjj

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[[Image:1sjj.gif|left|200px]]<br /><applet load="1sjj" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sjj" />
 
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'''Cryo-EM Structure of Chicken Gizzard Smooth Muscle alpha-Actinin'''<br />
 
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==Overview==
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==Cryo-EM Structure of Chicken Gizzard Smooth Muscle alpha-Actinin==
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Cryoelectron microscopy was used to obtain a 3-D image at 2.0 nm resolution of 2-D arrays of smooth muscle alpha-actinin. The reconstruction reveals a well-resolved long central domain with 90 degrees of left-handed twist and near 2-fold symmetry. However, the molecular ends which contain the actin binding and calmodulin-like domains, have different structures oriented approximately 90 degrees to each other. Atomic structures for the alpha-actinin domains were built by homology modeling and assembled into an atomic model. Model building suggests that in the 2-D arrays, the two calponin homology domains that comprise the actin-binding domain have a closed conformation at one end and an open conformation at the other end due to domain swapping. The open and closed conformations of the actin-binding domain suggests flexibility that may underlie Ca2+ regulation. The approximately 90 degrees orientation difference at the molecular ends may underlie alpha-actinin's ability to crosslink actin filaments in nearly any orientation.
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<StructureSection load='1sjj' size='340' side='right'caption='[[1sjj]], [[Resolution|resolution]] 20.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sjj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SJJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 20&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjj OCA], [https://pdbe.org/1sjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sjj RCSB], [https://www.ebi.ac.uk/pdbsum/1sjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sjj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ACTN1_CHICK ACTN1_CHICK] F-actin cross-linking protein is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sj/1sjj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sjj ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SJJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJJ OCA].
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*[[Actinin 3D structures|Actinin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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A 3-D reconstruction of smooth muscle alpha-actinin by CryoEm reveals two different conformations at the actin-binding region., Liu J, Taylor DW, Taylor KA, J Mol Biol. 2004 Apr 16;338(1):115-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15050827 15050827]
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[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Liu, J.]]
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[[Category: Liu J]]
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[[Category: Taylor, D W.]]
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[[Category: Taylor DW]]
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[[Category: Taylor, K A.]]
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[[Category: Taylor KA]]
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[[Category: 3-helix bundle]]
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[[Category: actin binding protein]]
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[[Category: calmodulin-like domain]]
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[[Category: calponin homology domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:02:10 2008''
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Current revision

Cryo-EM Structure of Chicken Gizzard Smooth Muscle alpha-Actinin

PDB ID 1sjj

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