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1sk9

From Proteopedia

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[[Image:1sk9.jpg|left|200px]]<br /><applet load="1sk9" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sk9, resolution 1.64&Aring;" />
 
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'''Crystallographic snapshots of Aspergillus fumigatus phytase revealing its enzymatic dynamics'''<br />
 
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==Overview==
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==Crystallographic snapshots of Aspergillus fumigatus phytase revealing its enzymatic dynamics==
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Understanding of the atomic movements involved in an enzymatic reaction needs structural information on the active and inactive native enzyme molecules and on the enzyme-substrate, enzyme-intermediate, and enzyme-product(s) complexes. By using the X-ray crystallographic method, four crystal structures of Aspergillus fumigatus phytase were obtained at resolution higher than 1.7 A. The pH-dependent catalytic activity of A. fumigatus phytase was linked to three water molecules that may prevent the substrate from binding and thus block nucleophilic attack of the catalytic imidazole nitrogen. Comparison of various structures also identified the water molecule that attacks the phosphamide bond during the hydrolysis process, and established the hydrolysis pathway of the intermediate. Additionally, two reaction product phosphates were observed at the active site, suggesting a possible product release pathway after hydrolysis of the intermediate. These results can help explain the catalytic mechanism throughout the whole acid phosphatase family, as all key residues are conserved.
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<StructureSection load='1sk9' size='340' side='right'caption='[[1sk9]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sk9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SK9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.64&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sk9 OCA], [https://pdbe.org/1sk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sk9 RCSB], [https://www.ebi.ac.uk/pdbsum/1sk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sk9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHYA_ASPFU PHYA_ASPFU] Catalyzes the hydrolysis of inorganic orthophosphate from phytate.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sk/1sk9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sk9 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SK9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus] with <scene name='pdbligand=NAG:'>NAG</scene> and <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-phytase 3-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.8 3.1.3.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SK9 OCA].
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*[[Phytase 3D structures|Phytase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystallographic snapshots of Aspergillus fumigatus phytase, revealing its enzymatic dynamics., Liu Q, Huang Q, Lei XG, Hao Q, Structure. 2004 Sep;12(9):1575-83. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15341723 15341723]
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[[Category: 3-phytase]]
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[[Category: Aspergillus fumigatus]]
[[Category: Aspergillus fumigatus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hao, Q.]]
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[[Category: Hao Q]]
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[[Category: Huang, Q.]]
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[[Category: Huang Q]]
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[[Category: Lei, X G.]]
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[[Category: Lei XG]]
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[[Category: Liu, Q.]]
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[[Category: Liu Q]]
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[[Category: NAG]]
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[[Category: PO4]]
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[[Category: big alpha/beta domain]]
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[[Category: catalytic dynamics]]
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[[Category: catalytic sites]]
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[[Category: product release pathway]]
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[[Category: small alpha domain]]
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[[Category: water structures]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:02:23 2008''
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Current revision

Crystallographic snapshots of Aspergillus fumigatus phytase revealing its enzymatic dynamics

PDB ID 1sk9

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