1std

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[[Image:1std.gif|left|200px]]<br /><applet load="1std" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1std, resolution 2.9&Aring;" />
 
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'''CRYSTAL STRUCTURE OF SCYTALONE DEHYDRATASE: A DISEASE DETERMINANT OF THE RICE PATHOGEN, MAGNAPORTHE GRISEA'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF SCYTALONE DEHYDRATASE: A DISEASE DETERMINANT OF THE RICE PATHOGEN, MAGNAPORTHE GRISEA==
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BACKGROUND: Rice blast is caused by the pathogenic fungus,-Magnaporthe grisea. Non-pathogenic mutants have been identified that lack enzymes in the biosynthetic pathway of dihydroxynapthalene-derived melanin. These enzymes are therefore prime targets for fungicides designed to control rice blast disease. One of the enzymes identified by genetic analysis as a disease determinant is scytalone dehydratase. RESULTS: The three-dimensional structure of scytalone dehydratase in complex with a competitive inhibitor has been determined at 2.9 A resolution. A novel fold, a cone-shaped alpha + beta barrel, is adopted by the monomer in this trimeric protein, burying the hydrophobic active site in its interior. The interactions of the inhibitor with the protein side chains have been identified. The similarity of the inhibitor to the substrate and the side chains involved in binding afford some insights into possible catalytic mechanisms. CONCLUSIONS: These results provide a first look into the structure and catalytic residues of a non-metal dehydratase, a large class of hitherto structurally uncharacterized enzymes. It is envisaged that a detailed structural description of scytalone dehydratase will assist in the design of new inhibitors for controlling rice blast disease.
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<StructureSection load='1std' size='340' side='right'caption='[[1std]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1std]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyricularia_grisea Pyricularia grisea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1STD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1STD FirstGlance]. <br>
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1STD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Magnaporthe_grisea Magnaporthe grisea] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=BFS:'>BFS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Scytalone_dehydratase Scytalone dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.94 4.2.1.94] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1STD OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BFS:N-[1-(4-BROMOPHENYL)ETHYL]-5-FLUORO+SALICYLAMIDE'>BFS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1std FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1std OCA], [https://pdbe.org/1std PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1std RCSB], [https://www.ebi.ac.uk/pdbsum/1std PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1std ProSAT]</span></td></tr>
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Crystal structure of scytalone dehydratase--a disease determinant of the rice pathogen, Magnaporthe grisea., Lundqvist T, Rice J, Hodge CN, Basarab GS, Pierce J, Lindqvist Y, Structure. 1994 Oct 15;2(10):937-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7866745 7866745]
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</table>
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[[Category: Magnaporthe grisea]]
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== Function ==
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[[Category: Scytalone dehydratase]]
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[https://www.uniprot.org/uniprot/SCYD_MAGO7 SCYD_MAGO7] Catalyzes two steps in melanin biosynthesis. From scytalone they are two dehydration steps and one reduction step to yield melanin.
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Lindqvist, Y.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Lundqvist, T.]]
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Check<jmol>
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[[Category: BFS]]
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<jmolCheckbox>
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[[Category: SO4]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/st/1std_consurf.spt"</scriptWhenChecked>
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[[Category: lyase (carbon-oxygen)]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:05:00 2008''
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1std ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Pyricularia grisea]]
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[[Category: Lindqvist Y]]
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[[Category: Lundqvist T]]

Current revision

CRYSTAL STRUCTURE OF SCYTALONE DEHYDRATASE: A DISEASE DETERMINANT OF THE RICE PATHOGEN, MAGNAPORTHE GRISEA

PDB ID 1std

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