D-xylose isomerase

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: {{STRUCTURE_4xis| PDB=4xis | SIZE=400| SCENE= |right|CAPTION=Mn-containing D-xylose isomerase complex with xylose, 4xis. }} '''D-xylose isomerase''' (DXI) catalyzes the conversion o...)
Current revision (10:07, 18 December 2022) (edit) (undo)
 
(26 intermediate revisions not shown.)
Line 1: Line 1:
-
{{STRUCTURE_4xis| PDB=4xis | SIZE=400| SCENE= |right|CAPTION=Mn-containing D-xylose isomerase complex with xylose, [[4xis]]. }}
+
<StructureSection load='' size='350' side='right' caption='Structure of D-xylose isomerase complex with D-xylose and Mn+2 ions (purple) (PDB code [[1xld]]).' scene='52/525128/Cv/1'>
 +
== Function ==
-
'''D-xylose isomerase''' (DXI) catalyzes the conversion of D-xylose (DX) to D-xylitol. DXI is part of the fructose and mannose metabolism pathways and is used commercially in the production of corn syrup. Sorbitol is a competitive inhibitor of DXI. DXI is a metal-ion activated tetramer.
+
'''D-xylose isomerase''' (DXI) catalyzes the conversion of D-xylose (DX) to D-xylitol. DXI is part of the fructose and mannose metabolism pathways. Sorbitol is a competitive inhibitor of DXI. DXI is a metal-ion activated tetramer.<ref>PMID:13421660</ref>
-
[[6xia]] – DXI – Streptomyces albus<br />
+
== Relevance ==
-
[[1xla]] – ArDXI – Arthrobacter<br />
+
-
[[3xin]], [[7xim]] - AmDXI - Actinoplanes missouriensis<br />
+
-
[[1bhw]] - AmDXI (mutant) <br />
+
-
[[1bxb]], [[1bxc]] - DXI – Thermus thermophilus<br />
+
-
[[2gub]], [[3kbj]], [[3kcj]] – SrDXI - Streptomyces rubiginosus<br />
+
-
[[3qza]] - SrDXI - neutron
+
-
===Mn-containing DXI===
+
DXI is used commercially in the production of high fructose corn syrup.
-
[[1xis]], [[1xib]], [[1xij]], [[2glk]], [[3n4a]] – SrDXI + Mn<br />
+
== Structural highlights ==
-
[[2gyi]] - SoDXI + Mn – Streptomyces olivochromogenos<br />
+
-
[[1xle]], [[1xlk]] - ArDXI + Mn<br />
+
-
[[1a0d]] - DXI + Mn – Bacillus stearothermophilus
+
-
==Mn-containing DXI complex==
+
<scene name='52/525128/Cv/7'>The biological assembly of D-xylose isomerase</scene> from ''Arthrobacter sp'' is homotetramer. DXI active site contains <scene name='52/525128/Cv/8'>2 Mn+2 ions</scene> and the <scene name='52/525128/Cv/9'>ligand binding pocket is composed of aromatic and acidic residues</scene> (water molecules shown as red spheres).<ref>PMID:2319597</ref>
 +
==3D structures of D-xylose isomerase==
 +
[[D-xylose isomerase 3D structures]]
-
[[4xis]], [[1xic]] - SrDXI + DX + Mn<br />
+
</StructureSection>
-
[[1xld]], [[1xlj]] - ArDXI + xylitol + Mn<br />
+
-
[[1xig]], [[3gnx]] - SrDXI + xylitol + Mn<br />
+
-
[[1s5n]] - SoDXI + xylitol + Mn<br />
+
-
[[1xii]] - SrDXI + xylulose + Mn<br />
+
-
[[8xia]] – SrDXI + DX + Mn<br />
+
-
[[9xia]] – SrDXI + fructose + Mn<br />
+
-
[[1xif]] - SrDXI + glucose + Mn<br />
+
-
[[1s5m]] - SoDXI + glucose + Mn<br />
+
-
[[1xid]] - SrDXI + ascorbate + Mn<br />
+
-
[[1xie]], [[1xih]] - SrDXI + sorbitol + Mn<br />
+
-
[[1did]] - ArDXI + glucitol + Mn<br />
+
-
[[1xlf]] - ArDXI + gluconic acid + Mn<br />
+
-
[[1xli]] - ArDXI + glucose derivative + Mn<br />
+
-
===Mg-containing DXI===
+
== References ==
 +
<references/>
-
[[1xlb]] - ArDXI + Mg<br />
+
[[Category:Topic Page]]
-
[[1xya]], [[1xyl]] - SoDXI + Mg<br />
+
-
[[3u3h]] - SrDXI + Mg<br />
+
-
+
-
==Mg-containing DXI complex==
+
-
 
+
-
[[1xyb]], [[1xym]] - SoDXI + glucose + Mg<br />
+
-
[[1xyc]] - SoDXI + fructose + Mg<br />
+
-
[[4xia]], [[5xim]] – ArDXI + sorbitol + Mg<br />
+
-
[[5xia]], [[1xlc]], [[1xlg]] – ArDXI + xylitol + Mg<br />
+
-
[[2xis]], [[4duo]] - SrDXI + xylitol + Mg<br />
+
-
[[3cwh]] - SrDXI + xylulose + Mg<br />
+
-
[[2xim]], [[1xin]] - AmDXI + xylitol + Mg<br />
+
-
[[5xin]], [[6xim]], [[8xim]], [[9xim]] - AmDXI + DX + Mg<br />
+
-
[[3xis]] - SrDXI + DX + Mg<br />
+
-
[[1die]] - ArDXI + moranoline + Mg<br />
+
-
 
+
-
===Co-containing DXI===
+
-
 
+
-
[[4xim]] - AmDXI + Co<br />
+
-
[[2a0c]] - DXI + Co – Thermoanaerobacterium<br />
+
-
[[1a0e]] - DXI + Co – Thermotoga neapolitana<br />
+
-
[[1qt1]] - SdDXI + Co – Streptomyces diastaticus<br />
+
-
[[2gve]], [[4a8i]], [[4a8l]], [[4a8r]], [[4a8n]] - SrDXI + Co<br />
+
-
 
+
-
==Co-containing DXI complex==
+
-
 
+
-
[[1xim]] - AmDXI + xylitol + Co<br />
+
-
[[3xim]], [[2xin]] - AmDXI + sorbitol + Co<br />
+
-
 
+
-
===Al-containing DXI===
+
-
 
+
-
[[1xlh]] - ArDXI + Al<br />
+
-
[[1xlm]] - ArDXI (mutant) + xylitol + Al
+
-
 
+
-
===Zn-containing DXI===
+
-
 
+
-
[[1xll]] - ArDXI + Zn
+
-
 
+
-
===Cd-containing DXI===
+
-
 
+
-
[[3kbs]] - SrDXI + Cd<br />
+
-
[[3kbm]], [[3kcl]] - SrDXI + glucose + Cd
+
-
 
+
-
===Ni-containing DXI===
+
-
 
+
-
[[3kbv]], [[3qys]] - SrDXI + Ni<br />
+
-
[[3kbn]], [[3kco]] - SrDXI + glucose + Ni<br />
+
-
[[4dvo]] - SrDXI + sorbitol + Ni
+
-
 
+
-
===Ca-containing DXI===
+
-
 
+
-
[[1gw9]] - SrDXI + DX + Ca
+
-
 
+
-
===Bi-metal-containing DXI===
+
-
 
+
-
[[1mnz]], [[1o1h]], [[2g4j]] - SrDXI + Mg + Ca<br />
+
-
[[1oad]], [[4e3v]] - SrDXI + Mg + Mn<br />
+
-
[[1muw]] - SoDXI + Mg + Mn<br />
+
-
[[1clk]] - SdDXI + Co + Mg<br />
+
-
[[3kbw]] - SrDXI + Ni + Mg<br />
+

Current revision

Structure of D-xylose isomerase complex with D-xylose and Mn+2 ions (purple) (PDB code 1xld).

Drag the structure with the mouse to rotate

References

  1. ↑ MARSHALL RO, KOOI ER. Enzymatic conversion of D-glucose to D-fructose. Science. 1957 Apr 5;125(3249):648-9. PMID:13421660
  2. ↑ Collyer CA, Henrick K, Blow DM. Mechanism for aldose-ketose interconversion by D-xylose isomerase involving ring opening followed by a 1,2-hydride shift. J Mol Biol. 1990 Mar 5;212(1):211-35. PMID:2319597

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

Retrieved from "http://52.214.119.220/wiki/index.php/D-xylose_isomerase"
Personal tools