1t1g

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[[Image:1t1g.jpg|left|200px]]<br /><applet load="1t1g" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1t1g, resolution 1.18&Aring;" />
 
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'''High Resolution Crystal Structure of Mutant E23A of Kumamolisin, a sedolisin type proteinase (previously called Kumamolysin or KSCP)'''<br />
 
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==Overview==
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==High Resolution Crystal Structure of Mutant E23A of Kumamolisin, a sedolisin type proteinase (previously called Kumamolysin or KSCP)==
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Kumamolisin, an extracellular proteinase derived from an acido/thermophilic Bacillus, belongs to the sedolisin family of endopeptidases characterized by a subtilisin-like fold and a Ser-Glu-Asp catalytic triad. In kumamolisin, the Asp82 carboxylate hydrogen bonds to Glu32-Trp129, which might act as a proton sink stabilizing the catalytic residues. The 1.2/1.3 A crystal structures of the Glu32--&gt;Ala and Trp129--&gt;Ala mutants show that both mutations affect the active-site conformation, causing a 95% activity decrease. In addition, the 1.2 A crystal structure of the Ser278--&gt;Ala mutant of pro-kumamolisin was determined. The prodomain exhibits a half-beta sandwich core docking to the catalytic domain similarly as the equivalent subtilisin prodomains in their catalytic-domain complexes. This pro-kumamolisin structure displays, for the first time, the uncleaved linker segment running across the active site and connecting the prodomain with the properly folded catalytic domain. The structure strongly points to an initial intramolecular activation cleavage in subtilases, as presumed for pro-subtilisin and pro-furin.
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<StructureSection load='1t1g' size='340' side='right'caption='[[1t1g]], [[Resolution|resolution]] 1.18&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1t1g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._MN-32 Bacillus sp. MN-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T1G FirstGlance]. <br>
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1T1G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._mn-32 Bacillus sp. mn-32] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1G OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.18&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1g OCA], [https://pdbe.org/1t1g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t1g RCSB], [https://www.ebi.ac.uk/pdbsum/1t1g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t1g ProSAT]</span></td></tr>
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1.2 A crystal structure of the serine carboxyl proteinase pro-kumamolisin; structure of an intact pro-subtilase., Comellas-Bigler M, Maskos K, Huber R, Oyama H, Oda K, Bode W, Structure. 2004 Jul;12(7):1313-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15242607 15242607]
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</table>
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[[Category: Bacillus sp. mn-32]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/Q8RR56_9BACI Q8RR56_9BACI]
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[[Category: Bode, W.]]
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== Evolutionary Conservation ==
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[[Category: Comellas-Bigler, M.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Huber, R.]]
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Check<jmol>
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[[Category: Maskos, K.]]
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<jmolCheckbox>
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[[Category: Oda, K.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t1/1t1g_consurf.spt"</scriptWhenChecked>
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[[Category: Oyama, H.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: CA]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: SO4]]
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</jmolCheckbox>
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[[Category: catalytic mechanism]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t1g ConSurf].
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[[Category: kumamolisin]]
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<div style="clear:both"></div>
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[[Category: sedolisin]]
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__TOC__
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[[Category: serine-carboxyl proteinases]]
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</StructureSection>
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[[Category: subtilases]]
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[[Category: Bacillus sp. MN-32]]
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[[Category: Large Structures]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:08:51 2008''
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[[Category: Bode W]]
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[[Category: Comellas-Bigler M]]
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[[Category: Huber R]]
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[[Category: Maskos K]]
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[[Category: Oda K]]
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[[Category: Oyama H]]

Current revision

High Resolution Crystal Structure of Mutant E23A of Kumamolisin, a sedolisin type proteinase (previously called Kumamolysin or KSCP)

PDB ID 1t1g

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