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1eb0

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[[Image:1eb0.gif|left|200px]]<br />
 
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<applet load="1eb0" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1eb0, resolution 1.85&Aring;" />
 
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'''CRYSTAL STRUCTURE OF BACILLUS PASTEURII UREE AT 1.85 A, PHASED BY SIRAS. TYPE I CRYSTAL FORM.'''<br />
 
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==Overview==
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==Crystal structure of Bacillus pasteurii UreE at 1.85 A, phased by SIRAS. Type I crystal form.==
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Bacillus pasteurii UreE (BpUreE) is a putative chaperone assisting the, insertion of Ni(2+) ions in the active site of urease. The x-ray structure, of the protein has been determined for two crystal forms, at 1.7 and 1.85, A resolution, using SIRAS phases derived from a Hg(2+)-derivative. BpUreE, is composed of distinct N- and C-terminal domains, connected by a short, flexible linker. The structure reveals the topology of an elongated, homodimer, formed by interaction of the two C-terminal domains through, hydrophobic interactions. A single Zn(2+) ion bound to four conserved, His-100 residues, one from each monomer, connects two dimers resulting in, a tetrameric BpUreE known to be formed in concentrated solutions. The, Zn(2+) ion can be replaced by Ni(2+) as shown by anomalous difference ... [[http://ispc.weizmann.ac.il/pmbin/getpm?11602602 (full description)]]
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<StructureSection load='1eb0' size='340' side='right'caption='[[1eb0]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1eb0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sporosarcina_pasteurii Sporosarcina pasteurii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EB0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eb0 OCA], [https://pdbe.org/1eb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eb0 RCSB], [https://www.ebi.ac.uk/pdbsum/1eb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eb0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UREE_SPOPA UREE_SPOPA] Involved in urease metallocenter assembly. Binds nickel. Probably functions as a nickel donor during metallocenter assembly.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/1eb0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eb0 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacillus pasteurii UreE (BpUreE) is a putative chaperone assisting the insertion of Ni(2+) ions in the active site of urease. The x-ray structure of the protein has been determined for two crystal forms, at 1.7 and 1.85 A resolution, using SIRAS phases derived from a Hg(2+)-derivative. BpUreE is composed of distinct N- and C-terminal domains, connected by a short flexible linker. The structure reveals the topology of an elongated homodimer, formed by interaction of the two C-terminal domains through hydrophobic interactions. A single Zn(2+) ion bound to four conserved His-100 residues, one from each monomer, connects two dimers resulting in a tetrameric BpUreE known to be formed in concentrated solutions. The Zn(2+) ion can be replaced by Ni(2+) as shown by anomalous difference maps obtained on a crystal of BpUreE soaked in a solution containing NiCl(2). A large hydrophobic patch surrounding the metal ion site is surface-exposed in the biologically relevant dimer. The BpUreE structure represents the first for this class of proteins and suggests a possible role for UreE in the urease nickel-center assembly.
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==About this Structure==
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Structural basis for Ni(2+) transport and assembly of the urease active site by the metallochaperone UreE from Bacillus pasteurii.,Remaut H, Safarov N, Ciurli S, Van Beeumen J J Biol Chem. 2001 Dec 28;276(52):49365-70. Epub 2001 Oct 15. PMID:11602602<ref>PMID:11602602</ref>
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1EB0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sporosarcina_pasteurii Sporosarcina pasteurii]] with ZN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: ZN. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EB0 OCA]].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural basis for Ni(2+) transport and assembly of the urease active site by the metallochaperone UreE from Bacillus pasteurii., Remaut H, Safarov N, Ciurli S, Van Beeumen J, J Biol Chem. 2001 Dec 28;276(52):49365-70. Epub 2001 Oct 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11602602 11602602]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 1eb0" style="background-color:#fffaf0;"></div>
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[[Category: Sporosarcina pasteurii]]
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[[Category: Beeumen, J.Van.]]
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[[Category: Ciurli, S.]]
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[[Category: Remaut, H.]]
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[[Category: Safarov, N.]]
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[[Category: ZN]]
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[[Category: putative ni-chaperone]]
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[[Category: urease accessory protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:09:52 2007''
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==See Also==
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*[[Urease accessory protein 3D structures|Urease accessory protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Sporosarcina pasteurii]]
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[[Category: Ciurli S]]
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[[Category: Remaut H]]
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[[Category: Safarov N]]
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[[Category: Van Beeumen J]]

Current revision

Crystal structure of Bacillus pasteurii UreE at 1.85 A, phased by SIRAS. Type I crystal form.

PDB ID 1eb0

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