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1eji

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[[Image:1eji.gif|left|200px]]<br />
 
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<applet load="1eji" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1eji, resolution 2.90&Aring;" />
 
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'''RECOMBINANT SERINE HYDROXYMETHYLTRANSFERASE (MOUSE)'''<br />
 
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==Overview==
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==RECOMBINANT SERINE HYDROXYMETHYLTRANSFERASE (MOUSE)==
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Serine hydroxymethyltransferase (SHMT) is a pyridoxal phosphate-dependent, enzyme that catalyzes the reversible conversion of serine and, tetrahydrofolate to glycine and methylenetetrahydrofolate. This reaction, generates single carbon units for purine, thymidine, and methionine, biosynthesis. The enzyme is a homotetramer comprising two obligate dimers, and four pyridoxal phosphate-bound active sites. The mammalian enzyme is, present in cells in both catalytically active and inactive forms. The, inactive form is a ternary complex that results from the binding of, glycine and 5-formyltetrahydrofolate polyglutamate, a slow tight-binding, inhibitor. The crystal structure of a close analogue of the inactive form, of murine cytoplasmic SHMT (cSHMT), lacking only the polyglutamate tail of, the ... [[http://ispc.weizmann.ac.il/pmbin/getpm?11063567 (full description)]]
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<StructureSection load='1eji' size='340' side='right'caption='[[1eji]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1eji]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EJI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EJI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PLG:N-GLYCINE-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YL-METHANE]'>PLG</scene>, <scene name='pdbligand=THF:5-HYDROXYMETHYLENE-6-HYDROFOLIC+ACID'>THF</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eji OCA], [https://pdbe.org/1eji PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eji RCSB], [https://www.ebi.ac.uk/pdbsum/1eji PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eji ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLYC_MOUSE GLYC_MOUSE] Interconversion of serine and glycine.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ej/1eji_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eji ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1EJI is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]] with PLG and THF as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Glycine_hydroxymethyltransferase Glycine hydroxymethyltransferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.2.1 2.1.2.1]]. Structure known Active Sites: I, II, III and IV. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EJI OCA]].
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*[[Serine hydroxymethyltransferase 3D structures|Serine hydroxymethyltransferase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of a murine cytoplasmic serine hydroxymethyltransferase quinonoid ternary complex: evidence for asymmetric obligate dimers., Szebenyi DM, Liu X, Kriksunov IA, Stover PJ, Thiel DJ, Biochemistry. 2000 Nov 7;39(44):13313-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11063567 11063567]
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[[Category: Large Structures]]
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[[Category: Glycine hydroxymethyltransferase]]
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Kriksunov IA]]
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[[Category: Kriksunov, I.A.]]
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[[Category: Liu X]]
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[[Category: Liu, X.]]
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[[Category: Stover PJ]]
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[[Category: Stover, P.J.]]
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[[Category: Szebenyi DME]]
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[[Category: Szebenyi, D.M.E.]]
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[[Category: Thiel DJ]]
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[[Category: Thiel, D.J.]]
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[[Category: PLG]]
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[[Category: THF]]
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[[Category: asymmetric dimer]]
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[[Category: pyridoxal 5'-phosphate]]
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[[Category: serine-glycine conversion]]
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[[Category: tetrahydrofolate]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:10:49 2007''
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Current revision

RECOMBINANT SERINE HYDROXYMETHYLTRANSFERASE (MOUSE)

PDB ID 1eji

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