1q8d

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[[Image:1q8d.png|left|200px]]
 
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{{STRUCTURE_1q8d| PDB=1q8d | SCENE= }}
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==The crystal structure of GDNF family co-receptor alpha 1 domain 3==
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<StructureSection load='1q8d' size='340' side='right'caption='[[1q8d]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1q8d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q8D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q8d OCA], [https://pdbe.org/1q8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q8d RCSB], [https://www.ebi.ac.uk/pdbsum/1q8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q8d ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GFRA1_RAT GFRA1_RAT] Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q8/1q8d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q8d ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glial cell line-derived neurotrophic factor (GDNF) binds to the GDNF family co-receptor alpha1 (GFRalpha1) and activates RET receptor tyrosine kinase. GFRalpha1 has a putative domain structure of three homologous cysteine-rich domains, where domains 2 and 3 make up a central domain responsible for GDNF binding. We report here the 1.8 A crystal structure of GFRalpha1 domain 3 showing a new protein fold. It is an all-alpha five-helix bundle with five disulfide bridges. The structure was used to model the homologous domain 2, the other half of the GDNF-binding fragment, and to construct the first structural model of the GDNF-GFRalpha1 interaction. Using site-directed mutagenesis, we identified closely spaced residues, Phe213, Arg224, Arg225 and Ile229, comprising a putative GDNF-binding surface. Mutating each one of them had slightly different effects on GDNF binding and RET phosphorylation. In addition, the R217E mutant bound GDNF equally well in the presence and absence of RET. Arg217 may thus be involved in the allosteric properties of GFRalpha1 or in binding RET.
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===The crystal structure of GDNF family co-receptor alpha 1 domain 3===
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The structure of GFRalpha1 domain 3 reveals new insights into GDNF binding and RET activation.,Leppanen VM, Bespalov MM, Runeberg-Roos P, Puurand U, Merits A, Saarma M, Goldman A EMBO J. 2004 Apr 7;23(7):1452-62. Epub 2004 Mar 25. PMID:15044950<ref>PMID:15044950</ref>
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{{ABSTRACT_PUBMED_15044950}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1q8d" style="background-color:#fffaf0;"></div>
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[[1q8d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q8D OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:015044950</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Bespalov, M M.]]
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[[Category: Bespalov MM]]
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[[Category: Goldman, A.]]
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[[Category: Goldman A]]
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[[Category: Leppanen, V M.]]
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[[Category: Leppanen VM]]
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[[Category: Merits, A.]]
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[[Category: Merits A]]
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[[Category: Puurand, U.]]
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[[Category: Puurand U]]
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[[Category: Runeberg-Roos, P.]]
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[[Category: Runeberg-Roos P]]
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[[Category: Saarma, M.]]
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[[Category: Saarma M]]
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[[Category: All-alpha]]
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[[Category: Cys-rich]]
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[[Category: Hormone-growth factor receptor complex]]
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Current revision

The crystal structure of GDNF family co-receptor alpha 1 domain 3

PDB ID 1q8d

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